Apertures objective lens

Figure 3.84. Voxel obtained by irradiation of a mixed urethane acrylate oligomer and a urethane acrylate monomer in the presence of a mixture of benzoyl cyclohexanol and morpholino phenyl amino ketones at 780 nm using a 150-fs pulsed Ti sapphire mode-locked laser operating at 76 MHz, where light was focused by a 1.4 numerical aperture objective lens (a) scanning electronic microscopic images of the voxel and (b) longitudinal and lateral voxel size as function of the exposure time. (From Ref. [580] with permission of the American Institute of Physics.)...

The threshold of the pulse energy to induce the laser tsunami is relatively low for a femtosecond laser compared with nanosecond and picosecond lasers. The laser tsunami expands to a volume of (sub pm)3 around the focal point, when an intense laser pulse is focused into an aqueous solution by a high numerical aperture objective lens. When a culture medium containing living animal cells is irradiated, they could be manipulated by laser tsunami. Mouse NIH 3T3 cells cultured on a substrate can be detached and patterned arbitrarily on substrates [36]. We have also demonstrated that the laser tsunami is strong enough to transfer objects with size of a few 100 pm [37], which is impossible by conventional optical tweezers because the force due to the optical pressure is too weak. In addition we demonstrated for the first time the crystallization of organic molecules and proteins in their super-saturated solution by laser tsunami [38-40]. 

In orthoscopic observations parallel rays are required because the optical property of liquid crystals is direction-dependent. Often a low-power, low aperture objective lens is used so that most of the light will travel through the sample in the same direction or nearly so. Otherwise the substage iris (D in Figure 4.4) is closed accordingly so that a sufficient approximation of the parallel condition is achieved. 

Figure 10 illustrates schematic drawing of objective-type TIRFM. A high numerical aperture objective lens is mounted on an inverted microscope. A laser beam is passed through a neutral density filter (ND) and a beam expander (BE) to adjust its power and diameter. When the laser polarized linearly is used, the polarization of the laser beam is converted from linear to circular by a quarter-wave plate (A/4). The laser beam is focused by a lens (L) on the back focal plane of the objective, so that specimens are illuminated uniformly with Koehler illumination. By shifting the position of the mirror (M) located between the lens (L) and dichroic mirror (DM), the path of the incident laser light is shifted from the center to the edge of the objective. At the center position, the microscope can be used as a standard epi-fluorescence microscope (Fig. 10b). 

Laser trapping is a technique to manipulate small sized materials, which was developed by Ashkin in 1970 [20, 21]. In this experiment, a laser beam is tightly focused by an objective lens with high numerical aperture (NA), and a dielectric... 

The complications of windows can be avoided by substituting small apertures above and below the sample to restrict the diffusion of gas molecules while allowing penetration of the electron beam. Typically, pairs of apertures are added above and below the sample, with differential pumping lines attached between them. In the early in situ experimentation, an ECELL system (69) could be inserted inside the EM column vacuum between the objective lens pole pieces. 

Fig. 14. Schematic of the basic geometry of the aperture system and objective lens pole pieces incorporating radial holes for differential pumping system in the novel atomic resolution-ETEM design of Gai and Boyes (85-90) to probe catalysis at the atomic level.

Dark-field illumination is classified into three types. The first one is for a microscope equipped with low numerical aperture (NA) objective lenses (see Fig. 1). To cast a shadow at the objective lens, a ring-slit as shown in Fig. IB is inserted into the light path. The second is for highNA (>0.5) objective lenses. Special, ready-made dark-field condensers or lenses are used for dark-field illumination. The third is independent... 

Fig. 1. Typical locations for CAM components, showing the photometer, 1 filter wheel, 2 monochromator, 3 shutter and aperture unit, 4 beam splitter, 5 accessories for polarized light such as a rotary analyzer and a compensator, 6 beam splitter for epi-excitation fluorescence, 7 objective lens, 8 stage, 9 substage condenser, 10 condenser aperture, 11 polarizer, 12 field aperture for photometry, 13 shutter, 14 primary illuminator, 15 arc lamp, 16 shutter, 17 monochromator, 18 filter wheel, 19 and ocular, 20.

In a confocal microscope, invented in the mid-1950s, a focused spot of light scans the specimen. The fluorescence emitted by the specimen is separated from the incident beam by a dichroic mirror and is focused by the objective lens through a pinhole aperture to a photomultiplier. Fluorescence from out-of-focus planes above and below the specimen strikes the wall of the aperture and cannot pass through the pinhole (Figure 11.3). 

The basic ECELL geometry consists of small apertures above and below the sample and the apertures are mounted inside the bores of the objective lens polepieces (figure 2.10(d)). The controlled environment ECELL volume is the normal sample chamber of the microscope. It is separated from the rest of the column by the apertures in each polepiece and by the addition of a gate valve, which is normally kept closed, in the line to the usual ion-getter pump (IGP) at the rear of the column. Differential pumping systems are connected between the... 

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