Antisense oligodeoxynucleotide

Standifer K, Chien C, Wahlstedt C, Brown G, Pasternak G. Selective loss of delta opioid analgesia and binding by antisense oligodeoxynucleotide to a delta opioid receptor. Neuron 1994 12 805-810. 

Alvarez-Salas, L.M., Benitez-Hess, M.L., and DiPaolo, J.A. 2003. Advances in the development of ribozymes and antisense oligodeoxynucleotides as antiviral agents for human papillomaviruses. Antiviral Therapy 8(4), 265-278. 

The translation of the sequence of the cDNA encoding deacetylvindoline 4-O-acetyltransferase compared to other putative plant acetyltransferases revealed a conserved region near the carboxy terminus of the proteins. This sequence was used to design a degenerate antisense oligodeoxynucleotide primer for PCR. The sense primer was based upon an internal peptide sequence of salutaridinol 7-0-acetyltransferase. This approach finally yielded a partial cDNA that encoded salutaridinol 7-O-acetyltransferase. The full-length clone was obtained by RACE-PCR and was functionally expressed in S. frugiperda Sf9 cells.28 The amino acid sequence of salutaridinol 7-O-acetyltransferase is most similar (37% identity) to that of deacetylvindoline acetyltransferase of C. roseus.27... 

Matsumoto, R.R. et al., Involvement of sigma receptors in the behavioral effects of cocaine evidence from novel ligands and antisense oligodeoxynucleotides, Neuropharmacology. 42, 1043, 2002. 

Wagner, J. A., T. V. McDonald, P. T. Nghiem, A. W. Lowe, H. Schulman, D. C. Gruenert, L. Stryer, and P. Gardner. 1992. Antisense oligodeoxynucleotides to the cystic fibrosis transmembrane conductance regulator inhibit cAMP-activated but not calcium-activated chloride currents. Proc Natl Acad Sci USA 89(15) 6785-9. 

Pasternak GW, Standifer KM. (1995). Mapping of opioid receptors using antisense oligodeoxynucleotides correlating their molecular biology and pharmacology. Trends Pharmacol Sci. 16(10) 344-50. 

Pugh G Jr, Abood ME, Welch SP. (1995). Antisense oligodeoxynucleotides to the kappa-1 receptor block the antinociceptive effects of delta 9-THC in the spinal cord. Brain Res. 689(1) 157-58. 

Rossi G, Pan YX, Cheng J, Pasternak GW. (1994). Blockade of morphine analgesia by an antisense oligodeoxynucleotide against the mu receptor. Life Sci. 54 375-79. 

Figure 5.12. Chemical structure of antisense oligodeoxynucleotides (AS-ODN). Phosphorothioate and methylphosphonate AS-ODN have a sulfur atom and a methyl group respectively, substituted for a nonbridging oxygen atom to increase stability to nucleases.

Pharmacokinetic Aspects of Antisense Oligodeoxynucleotides and Renal Distribution... 

DNA methyltransferase antisense oligodeoxynucleotide MG98 given as a 21-day continuous infusion every 4 weeks. Investigational New Drugs, 21, 85-97. 

Nemunaitis J, Holmlund JT, Kraynak M, Richards D, Bruce J, Ognoskie N, Kwoh TJ, Geary R, Dorr A, Von HoffD, Eckhardt SG (1999) Phase I ev uation of ISIS 3521, an antisense oligodeoxynucleotide ot protein kinase C-alpha, in patients with advanced cancer.) Clin Oncol 17 3586-3595... 

Cleek RL, Rege AA, Detmer LA, Eskin SG, Mikos AG. Inhibition of smooth muscle cell growth in vitro by an antisense oligodeoxynucleotide releases from poly(DL-lactic-co-glycolic acid) mictoparticles. J Biomed Mater Res 1997 35 525-530. 

Landgraf R, Gerstberger R, Montkowski A, Probst JC, Wotjak CT, Holsboer F, Engelmann M (1995) VI vasopressin receptor antisense oligodeoxynucleotide into septum reduces vasopressin binding, social discrimination abilities, and anxiety-related behavior in rats. J Neimosci 15 4250-4258... 

Wahlestedt C, Pich EM, Koob GF, et al Modulation of anxiety and neuropeptide Y-Yl receptors by antisense oligodeoxynucleotides. Science 259 528-531, 1993 Waka T, Fukada N Pharmacologic profile of a new anxiolytic, DN 2327 effect of R015-1788 and interaction with diazepam in rodents. Psychopharmacology 103 314-322, 1991... 

---