Antimicrobial assays liquid

McCann S.J., O. White, and B. Keevil (2002). Assay of teicoplanin in serum Comparison of high-performance liquid chromatography and fluorescence polarization immunoassay. Journal of Antimicrobial Chemotherapy 50 107-110. 

A preservative is a substance that extends the shelf-life of drug products by preventing oxidation or inhibiting microbial growth.14 Preservatives must be monitored in the products since they are considered to be active components. Generic HPLC assays are typically developed for preservatives such as buty-lated hydroxytoluene (BHT), an antioxidant for solid dosage forms, and antimicrobials such as parabens, sodium benzoate, or sorbic acid in liquid formulations. For these additive components, typical assay specifications are 85-115% of label claim. 

A wide variety of commercial LFIA kits for the detection of antibiotic residues is available and the most well-characterised of these are summarized in Table 5.4, including the rapid one-step assay (ROSA) range from Charm Sciences Inc., and the Tetrasensor, Twinsensor, Trisensor, and Sulfasensor from Unisensor SA and the Betastar from Neogen Corporation. Other LFIA assays have been reported in the scientific literature for the detection of antimicrobial residues, including a lateral-flow device for nicarbazin detection in animal feedstuffs. However, at present these are not commercially available. These LFIA tests incorporate either a receptor protein or an antibody as the specific capture molecule and operate in the competitive assay format (most applicable for small-molecule detection). The sample preparation protocols are based on either direct analysis of the liquid sample (e.g., milk) or a simple extraction step for solid or complex matrices using buffer(s) supplied in the test kit. In general, the time required to perform these tests is less than 30 min with only basic laboratory equipment, if any, required. 

Bogialli S, Curini R, Di Corcia A, et ah. Rapid confirmatory assay for determining 12 sulfonamide antimicrobials in milk and eggs by matrix solid phase dispersion and liquid... 

Berardi G, Bogialli S, Curini R, et ah. Evaluation of a method for assaying sulfonamide antimicrobial residues in cheese Hot water extraction and liquid chromatography-tandem mass spectrometry, J. Agric. Food Chem. 2006 54 4537-4543. 

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