Antihemolytic activity

Antihemolytic activity. Water extract of the dried seed, administered to rabbits red blood cells at variable concentrations, was inactive vs Staphylococcus aureus a-toxin-induced hemolysis and produced weak activity vs Vibrio parahaemolyticus-induced hemolysis . ... 

The antihemolytic activity of phenothiazines (log 1 /C) was also correlated with log K iAM (Tab. 3.2). Again, it was found that the corresponding equation with log Doct is of significantly lower statistical value ... 

Naim, M., Gestetner, B., Bondi, A., and Birk, Y. 1976. Antioxidative and antihemolytic activities of soybean isoflavones. J. Agric. Food Chem. 24 1174—1177. 

Several physiologic roles for CS have been postulated. First, it has been suggested that the presence of CS in erythrocytes (about 700 fig of CS are present in 100 ml erythrocytes [125]) stabilizes the erythrocyte membrane [130]. Thus, CS was found to reduce hemolysis up to 56% in hypotonic solutions, whereas several other steroid sulfates and cholesterol conjugates were devoid of antihemolytic activity [125]. Furthermore, the presence of CS had a critical influence on the disc shape of erythrocytes in hypotonic solution without CS, erythrocytes tended to become spherical and extend spicules [131]. A second postulated role of CS relates to spermatozoa. It has been suggested that CS stabilizes the membranes of these cells and that it may provide a structural trigger for capacitation [132,133]. Thus, CS is present in spermatozoa (15 fig/lO cells) as well as in seminal plasma, and appears to be concentrated in the acrosomal region [132]. On the other hand, sterol sulfatase activity is present in the human female reproductive tract [133]. There is no direct evidence, however, that CS contributes to the sperm membrane modification reactions that occur in association with fertilization. 

Naim M, Gestetner B, Bondi A, Birk Y. Antioxidative and antihemolytic activities of soybean isoflavone. J Agr Food Chem 1976 24 1174-1177. 

The biological activity of some chromandiones was studied with regard to their antihemolytic effect on blood (71JPS643). 2,2,7,8-Tetramethylchro-man-5,6-dione was shown to be a potent inhibitor of cyclic AMP phosphodiesterase in vitro (74BBA173 75MI1). 

Isoflavonoids also have been reported to have physiological activity in animal and human studies. Besides acting as an estro n mimic, it was reported that the isoflavones found in soybean seeds possess antihemolytic, antifungal, tumorsuppressing, and serum cholesterol-lowering effects. In addition, both epidemiological and dietary-intervention studies indicate that when isoflavones in soybean seeds and in subsequent protein products prepared from the seeds are part of the human dietary intake, those products provide many significant health benefits. - ... 

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