Antiglobulin response, antibody

Several humanized CD3 antibodies have been produced and tested, with the aim of reducing the adverse effects associated with the murine orthoclone antibody. The first results in renal transplant patients were encouraging, with promising clinical results, no evidence of antiglobulin response, and no or very few and minimal adverse effects attributable to various humanized monoclonal CD3 antibodies (16,17). In particular, careful patient monitoring failed to identify any significant cytokine release syndrome. 

G. L. DeNardo, B. M. Bradt, G. R. Mirick, and S. J. DeNardo, Human antiglobulin response to foreign antibodies therapeutic benefit Cancer Immunol Immunother 52(5) 309-316 (2003). 

J. D. Isaacs, The antiglobulin response to therapeutic antibodies. Semin Immunol 2 449-456 (1990). 

Dilhnan RO. Human antimouse and antiglobulin responses to monoclonal antibodies. Antibody Immunoconjug Radiopharm 1990 3 1-15. 

Antiglobulin tests, also called Coombs tests, indicate hemolytic anemia caused by an immune response. A direct Coombs test detects antibodies bound to erythrocytes, whereas an indirect Coombs test measures antibodies present in the serum. A positive finding in a direct Coombs test is usually indicative of antibody-mediated hemolysis. 

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