Antigenic determinant sequential

Studies of the specificities of experimentally induced antipolynucleotide antibodies have yielded some information on the antigenic determinants which could exist in a molecule of nucleic acid. Like the antibodies to proteins or synthetic polypeptides, antibodies to polynucleotides can be directed against different antigenic determinants derived from sequential regions as well as conformational factors in the molecule. Since antibodies in which the specificity is associated with the heterocyclic bases are obtained by use of single-stranded polynucleotides or certain components of nucleic acids it is thus evident that the bases themselves can be included among the antigenic determinants of polynucleotides. 

For re-ChIP (or double ChIP), two sequential rounds of immunoprecipitations are performed using two different antibodies. Re-ChIP allows one to determine whether two antigens occur together on the same chromatin fragment or on different fragments and can be used to assemble proteins in complexes [23]. 

The studies described above, as well as numerous others, have led to the conclusion that protein antigens may contain two general classes of determinant structures, namely, sequential and conformational. Sequential determinants would be those occurring in a linear conformation, as in the unfolded form of a protein, and conformational determinants would be those that are recognized by their homologous antibodies only when they occur in a particular conformation. The latter class would include determinants formed from amino acids that are located at distant points in the... 

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