Antibody, high-affinity binding

Before using the column to purify antibody-toxin conjugate, apply a solution of the RIP at a concentration of 1 mg/mL to block any high affinity binding sites. Remove the RIP using the elution procedure (see Note 13)... 

Immunocapture-polymerase chain reaction (IC-PCR) is a synthesis of two commonly used diagnostic tools. This method exploits the high-affinity binding of antibodies to provide a facile method of purification, usually from a complex matrix, supplying the substrate for PCR detection. PCR exponentially amplifies a deoxyribonucleic acid (DNA) template in a temperature-dependent fashion by the annealing of oligonucleotide primers, enzymatic extension of bound primers by a heat-stable polymerase, followed by denaturation of... 

Although assembled antigenic sites are more numerous, both sites are topographic in that they are composed of structures on the protein surface. However, an antibody exerts high-affinity binding to segmental sites only when those sites are in a preferred conformation. Therefore, all determinants must be conformation specific. 

Most ofthe clinical and ex vivo studies have focused on the hENTl transporter in lymphoproliferative malignancies because hENTl is the most abundant and widely distributed NTs in mammalian cells. Moreover, its abundance could be measured in the absence of suitable antibodies by high-affinity binding of NBTI or fluorescent nucleoside derivative SAENTA. The number of ENTl transporters determined by NBTI binding in acute myeloid and lymphoid leukemia (AML and ALL) cells exhibited interpatient variation and correlated with intracellular accumulation of the cytarabine metabolite ara-CTP [124]. Similarly, Gati et al. demonstrated a correlation between the expression of hENTl and the in vitro sensitivity to cytarabine and fiudarabine of blasts from acute leukemia patients [125, 138]. In contrast. 

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