Antibody fragments and genetically engineered constructs

The effective use of radiolabelled antibodies for any purpose, but especially for use in patients, requires that they are of the quality necessaiy for their intended application. Most importantly they should contain low levels of radioactive and non-radioactive impmities, the radiolabel should be bound to the antibocfy with a stability which ensures that it will not dissociate during the conditions of its 

Radiolabelling efficiency TLC/HPLC As high as routinely possible 

Stability in solution 24 h storage in solution at 20 °C 5% loss in purity 

Serum stability 4 h incubation in fresh serum at 37 °C 10% loss in purity 

Antigen binding Direct radioligand binding assay 70% immunoreactive fraction 

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