Antibodies to dinitrophenol

In addition to eliciting a contact hypersensitivity response, benzo[a]pyrene has been shown to suppress this response, to other sensitizers. The effects of dermally applied benzo[a]pyrene (alone or following dermal pretreatment with the prostaglandin synthetase inhibitor, indomethacin) on contact hypersensitivity (cell-mediated immunity), and production of antibodies to dinitrophenol (DNP) (humoral immunity) were studied in male BALB/c mice treated for 6 weeks to 6 months (Andrews et al. 1991a). A group of mice treated with acetone served as controls. Benzo[a]pyrene alone caused a significant reduction (p<0.01) in the contact hypersensitivity response to dinitrofluorobenzene (DNFB) as measured by increases in ear thickness when compared to the vehicle controls. However, indomethacin pretreatment prevented the benzo[a]pyrene-induced contact hypersensitivity response. Benzo[a]pyrene also reduced antibody titres to DNP in treated mice. This suppressive effect on humoral immune function was not restored by pretreatment with indomethacin. These findings led the authors to conclude that the mechanism of... 

Figure 7.15 The relationship between the association equilibrium constants, K, and the dissociation rate constants, kr, for a series of antibodies to dinitrophenol (DNP).

Photoisomerizable dinitrophenyl spiropyran linked to a gold electrode by an aminoethanethiol spacer Antibody to dinitrophenol [3]... 

Recently, an examination of the binding kinetics of Rhodamine G-Iabeiled antibodies to dinitrophenol, also immobilized to a quartz light guide, was reported 91]. In a similar experiment, Rhodamine G-labelled IgG and insulin were shown to adsorb non-speciircally to serum albuminfused silica with both reversible and irreversible components. The characteristic time of the most rapidly reversible component measured was ca S ms and was limited by the rate of bulk diffusion. The binding was followed by fluorescence which, collected by a microscope from a ca S-pm surface area, spontaneously fluctuates as the solute molecules randomly bind to, unbind from, and/or diffuse along the surface in chemical equilibrium. 

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