Antibodies to Blood Group

Wiskott-Aldrich syndrome (WAS) Mutation on X chromosome involved in coding for protein regulating microvesicle formation. Complete absence of antibodies to blood group antigens. Small defective platelets, fiirombocytopenia and immunodeficiency. 

In a review, a comparison has been made of the specific combining sites of a number of lectins and those of antibodies to blood-group substances. A comprehensive review of both plant and animal lectins has been published. The application of lectins for the purification and characterization of enzymes and other proteins has been reviewed in order to define some of the variables that affect the binding of glycoproteins to lectins. The role of the oligosaccharides of cell-wall surface glycoproteins in variants of plasma membranes of mammalian cells that are resistant to cytotoxic lectins has been assessed. ... 

Human monoclonal antibodies to blood group antigens... 

HUMAN MONOCLONAL ANTIBODIES TO BLOOD GROUP ANTIGENS... 

Elevation of bilirubin commonly occurs in newborns. Sometimes this is not due to a deficiency in glucuronyl transferase but to blood group antigen-antibody incompatibilities which result in excess red cell destruction. Here, too, there is a rise in unconjugated bilirubin. Phototherapy and blood transfusion may both be helpful here. 

Immobilization of A and B blood group oligosaccharide haptens and preparation of immunoadsorbents with specificity to anti-A and anti-B antibodies has been carried out with the use of poly acrylate-coated PG (WPG-PA) [124]. Prespacered A and B-trisaccharide-fl-aminopropylglycosides were used for the synthesis. WPG-PA (1 g) quantitatively binds both haptens (2 pinole) whereas some other activated affinity supports (for example, CNBr-Sepharose 4B) do not. On the other hand, glycidoxypropyl-silica binds prespacered haptens completely but these materials reveal no specific adsorptivity. 

Biologicals. Figure 1 The ABO blood group system with immunochemical specificity due to terminal sugars and reciprocal antibodies in the serum. From Immunity Immune Response in Inflammatory Disease by DeFranco, Locksley and Robertson [2]. 

To study the binding mode of the yS-D-galactopyranosyl residue of the Lewis b human blood-group determinant to its monoclonal antibody or to a lectin, methyl 2-acetamido-2-deoxy-3-0-(6-deoxy-6-fluoro-2-0-a-L-fuco-pyranosyl-y -D-galactopyranosyl)-4-0-a-L-fucopyranosyl-/ -D-glucopyrano-side was synthesized. ... 

Dissolve the antibody to be modified at a concentration of l-10mg/ml in 0.1M sodium phosphate, 0.15M NaCl, pH 7.2, containing 10 mM EDTA. High levels of EDTA often are required to stop completely metal-catalyzed oxidation of sulfhydryl groups when working with serum proteins—especially polyclonal antibodies purified from antisera. Presumably, carry-over of iron from partially hemolyzed blood is the contaminating culprit. 

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