Antibodies nuclear receptors

Dual-label TR-FRET substrates have also been used for simultaneous monitoring of the enzymes related to apoptosis caspases 1, 3, and 6 [44]. Kokko et al. utilized fluorescent terbium chelates as the donors for two different acceptors, Alexa Fluor 488 and 680, in a dual-label immtmoassay of free and total PSA [45]. Free, uncomplexed PSA were measured with TR-FRET from terbium labeled (universal) antibody to free-specific Alexa 488 labeled antibody, and the total PSA was quantified with TR-FRET from terbium to another generic antibody labeled with Alexa 680. Kupcho et al. followed nuclear receptor coactivator recruitment... 

Gaub, M. P, Lutz, Y, Ruberte, E., Petkovich, M., Brand, N., and Chambon, P. (1989) Antibodies specific to the retinoic acid human nuclear receptors a and P Proc Natl. Acad Sci. USA 86, 3089-3093... 

Double-label ICC is another approach using a similar principle. It pairs a nuclear antibody with a cytoplasmic antibody on the same sample, such as keratin and estrogen receptor. At present, this method is mostly used in the research field. 

In the 1980s, Jensen and others obtained monoclonal antibodies against several of the nuclear hormone receptors (Dfaz-Chico et al. 1988 Jordan et al. 1990). These antibodies permitted the introduction of immunohistochemical techniques in the study of receptors. Consequently, King and Greene verified... 

A8. Alarcon-Segovia, D., Ruiz-Argiielles, A., and Fishbein, E., Antibody to nuclear ribonucleopro-tein penetrates live human mononuclear cells through Fc receptors. Nature 271, 67-69 (1978). 

Y2. Yanase, K., Smith, R. M., Puccetti, A., and Jarett, L., Madaio, M. P., Receptor-mediated cellular entry of nuclear localizing anti-DNA antibodies via myosin 1. J. Clin. Invest. 100,25-31 (1997). 

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