Antibodies isoforms

Marker source Upper pi Lower pi Group Average antibody isoform pi values ... 

P. G. Righetti, E. Wenisch, A. Jnngbaner, H. Katinger, and M. Faupel, Preparative purification of human monoclonal antibody isoforms in a multi-compartment electrolyser with immobiUne membranes, J. Chromatogr. 500 (1990), 681-696. 

Lubarskaya Y, Houde D, Woodard J, et al. (2005). Analysis of recombinant monoclonal antibody isoforms by electrospray ionization mass spectrometry as a strategy for streamlining characterization of recombinant monoclonal antibody charge heterogeneity. Anal. Biochem. Available sciencedirect.com. 

In some cases, the anionic POs adsorbed on chitin have similar antigenic determinants, but the plants belonging to different families - and even members of the same family - could have polysaccharide-specific POs with different structures. Thus, the majority of investigated species had anionic chitin-specific peroxidises, and these isoforms from potato Solcmaceae) and horseradish Brassicaceae) formed lines of precipitation with antibodies to wheat chitin-bound PO but not to anionic isoPO (Maksimov et al., 2000). However, protein extracts from several plants of Brassicaceae, Cucurhitaceae and Fahaceae formed precipitate with both the chitin-specific and anionic PO of wheat (Fig. 3). It was foimd that the greatest homology showed in plants and formed precipitation lines with the anionic PO of wheat (Tab. 2). 

In most plant materials that have been examined, several PME isoforms have been extracted fixDm the cell walls. What is their reqjective fimction How is their synthesis regulated -Immunolocalisation experiments have shown that some Golgi vesicles can be labelled with the JIM 5 antibody, which is known to recognize low-ester pectins [19]. This raises the question of the action pattern of the pectin methyltransferases. Can they produce pectins with different... 

The / 2-subunit isoform seems to have a function as a recognition element for cell adhesion in the brain. This association may also mediate fine regulation of transport because antibodies to the pi subunit (AMOG = association molecule of glia) cause dissociation of cell-cell adhesion and stimulate Na,K-transport [51]. 

With the arrival of new information on the amino acid sequence of various Ca " -ATPase isoforms [8,9,11,32,53-57,295] it became possible to identify the epitopes for the various antibodies, and to relate their positions to the three-dimensional structure of Ca -ATPase emerging from crystallographic studies [90,91,141,142,156,... 

Hydrophobic interaction chromatography (HIC) can be considered to be a variant of reversed phase chromatography, in which the polarity of the mobile phase is modulated by adjusting the concentration of a salt such as ammonium sulfate. The analyte, which is initially adsorbed to a hydrophobic phase, desorbs as the ionic strength is decreased. One application demonstrating extraordinary selectivity was the separation of isoforms of a monoclonal antibody differing only in the inclusion of a particular aspartic acid residue in the normal, cyclic, or iso forms.27 The uses and limitations of hydrophobic interaction chromatography in process-scale purifications are discussed in Chapter 3. 

In E. Coli bacterial lysates, the proteome (i.e., the full array of proteins produced) was analyzed by isoelectric focusing and mass spectrometry.97 A comparison of capillary electrophoretic separation and slab gel separation of a recombinant monoclonal antibody demonstrated that the precision, robustness, speed, and ease-of-use of CE were superior.98 Seventy-five proteins from the yeast ribosome were analyzed and identified by capillary electrophoresis coupled with MS/MS tandem mass spectrometry.99 Heavy-chain C-terminal variants of the anti-tumor necrosis factor antibody DE7 have been separated on capillary isoelectric focusing.100 Isoforms differing by about 0.1 pi units represented antibodies with 0,1 or 2 C-terminal lysines. 

Human myometrium has been demonstrated to contain the mRNA of all three isoforms of the ryanodine receptor, although type 1 and 3 mRNA are the most abundant (Martin et al 1999). Similarly, mRNA encoding the three types of InsP3 receptor are present in human myometrium (Morgan et al 1996). In cultured human myocytes, a lacy network can be outlined by using antibodies against... 

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