Antibodies immunoglobins

At moderately acidic pH values, the short chain fatty acid, caprylic acid, is an effective protein precipitant. It has found an application in immunoglobulin purification as conditions have been developed that result in the precipitation of the majority of non-immunoglobin proteins in the serum leaving the antibodies in solution. The concentration of caprylic acid required differs for the serum of different species as shown in Table 1 (see Note 12). 

Alkaline phosphatase is coupled to immunoglobin G antibody in a one-step procedure using the homobifunctional reagent glutaral-dehyde, which reacts with amino groups in the two proteins. The procedure is simple to perform and requires minimal equipment. 

The most common method of labeling immunoglobin G (IgG) antibody with this enzyme uses the homobifunctional reagent glu-... 

The isothiocyanate derivatives of fluorescein and rhodamine are widely used to label antibodies. They react with the amino groups of the immunoglobin G (IgG) molecule under alkaline conditions and a molar excess of about 20 is usually optimal. More recently, N-... 

IgE IgG IgM IL KLa KLH mAb PEG S-S TK tRNA Immunoglobin class E Immunoglobin class G Immunoglobin class M Interleukin Volumetric oxygen transfer coefficient keyhole limpet haemocyanin Monoclonal antibody polyethylene glycol Disulfide bridge thymidine kinase transcript of the RNA... 

Anti-G3PDH rabbit polyclonal antibody (Trevigen, Gaithersburg, MD, USA) Horseradish peroxidase linked anti-mouse or anti-rabbit immunoglobin (Amer-sham Pharmacia Biotech, Piscataway, NJ, USA) Triton X-100 (Bio-Rad, Hercules, CA, USA) aprotinin and leupeptin (Roche, Mannheim, Germany). 

The data show that the drug serves as an adjuvant for a variety of immunoglobin classes (IgG, IgM and IgE antibody production). The effects on IgA responses to antigenic stimulation remain still to be determined. 

Brown R, Kertiles L, Kleinmann R. Choice of immunoglobin G purification method in assays for antibodies to the thyrotropin receptor. Clin Chem 1986 32 2034-9. 

Ricin is a highly immunogenic toxin, and paired acute and convalescent sera should be obtained from survivors for measurement of antibody response. Conceptually, the detection of circulating antiricin antibodies formed in response to sublethal toxin exposure is possible for exposed individuals who survive for 2-3 weeks (Hunt et al., 1918). However, humoral immunoglobin M responses would likely be of short duration, and no immunological memory would be anticipated without boosting. 

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