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Antibodies glutaraldehyde

Glutaraldehyde was one of the first and still is one of the most commonly used crosslinking agents available for creating antibody-enzyme conjugates. The crosslinking process using... [Pg.797]

In a fume hood, add 10 pi of 25 percent glutaraldehyde (Sigma) per ml of antibody/enzyme solution. Mix well. [Pg.798]

Figure 20.7 Glutaraldehyde antibody-enzyme crosslinking procedures usually produce a wide range of high-molecular-weight complexes, some of which may precipitate from solution. Figure 20.7 Glutaraldehyde antibody-enzyme crosslinking procedures usually produce a wide range of high-molecular-weight complexes, some of which may precipitate from solution.
Despite the obvious disadvantages of glutaraldehyde-mediated conjugation, the crosslinker continues to be used to form enzyme-antibody complexes and in other applications. Many diagnostic tests still utilize antibody-enzyme conjugates prepared through glutaraldehyde... [Pg.966]

Ford, D.J., Radin, R., and Pesce, A.J. (1978) Characterization of glutaraldehyde coupled alkaline phosphatase-antibody and lactoperoxidase-antibody conjugates. Immunochemistry 15, 237. [Pg.1063]

Imagawa, M., Yoshitake, S., Hamguchi, Y., Ishikawa, E., Niitsu, Y., Urushizaki, I., Kanazawa, R., Tachibana, S., Nakazawa, N., and Ogawa, H. (1982) Characteristics and evaluation of antibody-horseradish peroxidase conjugates prepared by using a maleimide compound, glutaraldehyde, and periodate./. Appl. Biochem. 4, 41-57. [Pg.1076]

Five immobilization matrices have been tested and compared. These include a dextran layer on the sensor surface, succinic anhydride modified surface, monomer glutaraldehyde activated surface, polyglutaraldehyde activated surface and strepta-vidine/biotinylated antibody coated surface. [Pg.431]

See other pages where Antibodies glutaraldehyde is mentioned: [Pg.23]    [Pg.25]    [Pg.569]    [Pg.242]    [Pg.160]    [Pg.171]    [Pg.32]    [Pg.396]    [Pg.397]    [Pg.398]    [Pg.398]    [Pg.215]    [Pg.260]    [Pg.32]    [Pg.134]    [Pg.235]    [Pg.235]    [Pg.268]    [Pg.788]    [Pg.798]    [Pg.798]    [Pg.810]    [Pg.813]    [Pg.962]    [Pg.964]    [Pg.965]    [Pg.65]    [Pg.264]    [Pg.361]    [Pg.364]    [Pg.381]    [Pg.386]    [Pg.417]    [Pg.425]    [Pg.431]    [Pg.431]    [Pg.241]    [Pg.11]    [Pg.100]    [Pg.101]    [Pg.5]    [Pg.250]   
See also in sourсe #XX -- [ Pg.797 , Pg.798 ]

See also in sourсe #XX -- [ Pg.470 ]

See also in sourсe #XX -- [ Pg.470 ]



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