Glutaraldehyde 5 percent

Add a quantity of glutaraldehyde equal to a 10-fold molar excess over the amount of amines to be modified. A typical concentration of glutaraldehyde in the reaction mixture is 1.25 percent. In some cases, trial experiments will have to be done to check for solubility of the resultant modified protein. Scale back the quantity of glutaraldehyde added if precipitation occurs. 

After the final wash, suspend the particles in coupling buffer containing 10 percent glutaraldehyde and mix well to dissolve. 

Add fresh glutaraldehyde to the peptide/carrier solution to obtain a 1 percent final concentration. Mix well. 

In a fume hood, add 10 pi of 25 percent glutaraldehyde (Sigma) per ml of antibody/enzyme solution. Mix well. 

Add glutaraldehyde to this suspension to obtain a final concentration of 1.25 percent. 

JACHUK, S.J., BOUND, C.L., STEAL, J. BLAIN, P.G. (1989) Occupational hazard in hospital staff exposed to 2 percent glutaraldehyde in an endoscopy unit. Journal of the Society of Occupational Medicine, 39, 69-71. 

The results for the methylcellulose-collagen fibers in the previous section suggest that a maximum should be reached in the UTS vs. methylcellulose concentration curve. Figure shows that this maximum is at five percent methylcellulose for all glutaraldehyde cross-linking bath concentrations. Examination of stress-strain curves reveals that the modulus also peaks at five percent methylcellulose. 

For a cross-linking time of three minutes the optimum glutaraldehyde concentration is 0.5 for both collagen and five percent... 

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