Antibiotics, lactam membrane interaction

Takahashi K, Nakamura N, Terada T, et al. Interaction of beta-lactam antibiotics with H+/peptide cotransporters in rat renal bmsh-border membranes. J Pharmacol Exp Ther 1998 286 1037-1042. 

Carrier-mediated transport involves interaction of the drug with a specific transporter or carrier, in which drug is transferred across the cell membrane or entire cell and then released from the basal surface of the enterocyte into the circulation. The process is saturable and utilized by small hydrophilic molecules. Drugs that are shown to be transported by this mechanism include p-lactam antibiotics, cephalosporins, and ACE inhibitors. 

Extensive investigations of the properties of PBPs of E. coli, strain K12, were carried out. The results are indicative of our present understanding of PBPs and will be outlined. It should be stated that it is now understood that penicillin-sensitive enzymes such as DD-Cbase, peptidoglycan transpeptidase, and endopeptidases identified earlier are almost certainly identical with the PBPs under discussion here. Multiple PBPs have been discovered in all bacterial membranes studied. It is also now apparent that the interactions of (3-lactam antibiotics with bacteria can result in one or more effects on the physiology and structure of the cell. Thus inhibition of cell division can be observed so can lysis, bulge formation, or even the development of ovoid cell forms stable to osmosis. 

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