Affinity Chromatography and Immunoadsorption

Pack modified gel into column and adsorb sample containing a mixture of components A, C, and D  

Affinity chromatography can be applied to the isolation and purification of virtually all biological macromolecules. It has been used to purify nucleic acids, enzymes, transport proteins, antibodies, hormone receptor proteins, drug-binding proteins, neurotransmitter proteins, and many others. 

Successful application of affinity chromatography requires careful design of experimental conditions. The essential components, which are outlined below, are (1) creation and preparation of a stationary matrix with immobilized ligand and (2) design of column development and eluting conditions. 

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Considerable effort continues to be devoted to the development of new methods of enzyme immobilization and of novel affinity chromatography media. Immunoadsorption has been applied to the isolation of numerous biologically important molecules. Oligosaccharides co-polymerized with acrylamide have been exploited with much success for the isolation of many lectins from different sources. Active immobilized cells have been prepared and their potential applications for industrial processes and in medicine have been demonstrated. 

Polysaccharide carriers have been widely used for the preparation of immuno-adsorbents. Techniques and instrumentation for preparative immunoadsorptive separations have been discussed. The application of reversed immunosorbents to the immobilization of specific antibodies has been outlined, and a review of the use of affinity chromatography for the isolation of im-munologically reactive cells has appeared. ... 

Purification by affinity chromatography of membrane receptors for Pal and Pa2 Purification of anti-(picryl T suppressor factor) antibody by immunoadsorption Purification of retinol-binding protein by affinity chromatography... 

The repeated use of derivatives of agarose cyclic imidocarbonate in affinity chromatography has been discussed from the viewpoints of the nature of the binding of the protein to the affinant and the methods for regenerating used columns, etc. Agarose cyclic imidocarbonate has been used in automated immunoadsorption and affinity chromatographies. ... 

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