Anti-a-D-mannose antibodies

Anti-a-D-mannose antibodies have been isolated from the serum of rabbits immunized with the glycoconjugate of a-D-mannose and bovine serum albumin [85], The antibodies were purified by affinity chromatography with adsorption on a mannosyl-Sepharose column and elution with a-D-mannose or methyl a-D-mannoside. Such antibodies should be especially useful for studying the detection of diseases due to the appearance of abnormal glycoproteins that contain mannose polymers. 

Fig. 13.—A Agar diffusion of immune sera (Se), 1-thio-D-mannose antibodies (A ), and anti-BSA antibodies (A2) against Man-S-BSA (/) and BSA (2). B Agar-diffusion plate of anti-Man-S-antibodies and antibodies oxidized by peroxypropanoic acid for 0, 4, and 8 h. C, D, E, and F Hapten inhibition by agar diffusion, A = purified anti-Man-S antibodies I = antibodies + p-nitrophenyl 1 -thio-a-D-mannopyranoside 12 = antibodies + D-mannose I3 = antibodies + ethyl 1-thio-a-o-mannoside 1 to 6, outer wells contain decreasing concentration of Man-S-BSA. (Reprinted with permission from Journal of Protein Chemistry, Volume 9, J. H. Pazur, B. Liu, Nan Q Li, and Y. C. Lee, pp. 143-150, copyright 1990 Journal of Protein Chemistry.)...

Diffusion in agar performed by the conventional method shows a strong reaction between the antibody and Man-BSA, but not with BSA (Fig. 12, plates A). However, both compounds give precipitin complex with anti-BSA serum. Oxidation of the antigens with periodate no longer gives a precipitin reaction with the anti-mannose antibodies, but has no effect on the anti-BSA antibodies. Hapten-inhibition results are also shown in Fig. 12, plates C and D. These tests are conducted with the purified antibody... 

Fig. 12.—Reactivity of anti-a-mannose antibodies and anti-BSA antibodies with the native and periodate-oxidized mannose-glycoconjugate and BSA (plates A and B). M = Man-BSA, xM = periodate-oxidized Man-BSA, B = BSA, xB = periodate-oxidized BSA, A = antimannose antibodies, A2 = anti-BSA antibodies. Plates C and D show hapten inhibition of purified anti-mannose antibodies (A ) with mannose (L). The amount of antigen in the outer wells ranges from 20 /u.g in well 1 to 1 /u.g in well 6. [Reprinted with permission from J. H. Pazur, B. Liu, and T. Witham. J. Protein Chem., 13 (1994) 59-66.]...

Fig. 35.—Agar diffusion of xanthan antibodies (Ab) and chemically modified xanthan and inhibitors. A xanthan (well 1) reduced xanthan (well 2) oxidized xanthan (well 3) deacety-lated xanthan (well 4) enzymatically treated xanthan (well 5). B Xanthan with 6% pyruvate (well 1), xanthan with 4% pyruvate (well 2), xanthan with 2.8% pyruvate (well 3), xanthan with 0.5% pyruvate (well 4), and xanthan with 0.2% pyruvate (well 5). C, D, E Inhibition of anti-xanthan antibodies by glucuronic acid and mannose wells 1-6 contain decreasing concentrations of xanthan (1-0.3%) solutions of xanthan In antibody + glucuronic acid, In2 antibody + mannose. (Reprinted from Carbohydrate Polymers, Volume 27, J. H. Pazur, F. J. Miskiel, and N. T. Marchetti, pp. 85-91, copyright 1995, with permission from Elsevier Science Ltd., The Boulevard, Langford Lane, Kidlington, 0X5 1GB, UK.)...

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