8-anilino-l-naphthalenesulfonic

The binding of 8-anilino-l-naphthalenesulfonate (ANS) to ciliary dynein ATPase resulted in a marked increase in the dye s fluorescence intensity, accompanied by a blue shift in the observed fluorescence emission maximum" (Fig. 6). While dynein has 37 3 dye binding... 

A Study of Mixed Aqueous Solutions of Hydrocarbon and Fluorocarbon Surfactants Using 8-Anilino-l-naphthalenesulfonic Acid Ammonium Salt... 

A simple plot of FJF against [H] from titration of the quenching host into a guest solution should yield a straight line of slope Kn. Common fluorescent guests such as 8-anilino-l-naphthalenesulfonate (ANS, 1.7) may be used to probe complexation ability of various hosts in this way. 

In competitive homogeneous immunoassay, separation and quantitation of free and bound labeled antigen (cortisol) were carried out in a fused silica chip. Since the antibody-antigen complex was not detected, an internal standard (fluorescein) was added to aid quantitation. In addition, since most of the total cortisol was bound in the serum, a releasing agent, 8-anilino-l-naphthalenesulfonic acid (ANS), should be added [1006]. In other reports, competitive immunoassay for BSA was demonstrated after performing a CE separation on-chip [105,1005]. 

Ploug M, Ellis V, Dano K. Ligand interaction between urokinase-type plasminogen activator and its receptor probed with 8-anilino-l-naphthalenesulfonate. Evidence for a hydrophobic binding site exposed only on the intact receptor. Biochemistry 1994 33(30) 8991-8997. 

Other disazo dyes with good substantivity and high wet-fastness properties on polyamides are Acid Red 114 (40), made by coupling 0-tolidine to phenol which is then coupled to G-acid, followed by reaction of the phenolic hydroxyl group withy>-toluenesulfonyl chloride, and Acid Blue 113 (41) (metanilic acid — 1-naphthyl amine — 8-anilino-l-naphthalenesulfonic acid). 

Sodium measurements use three components for the reagent phase [73] an ionophore selective for Na immobilized on silica, an anionic fluorophore (F), the ammonium salt of 8-anilino-l-naphthalenesulfonic acid and a cationic polyelectrolyte (P), copper(II)-poly-... 

Crown ether-capped jS-CyD 71 showed a binding afEnity for 8-anilino-l-naphthalenesulfonate (AN S) 88 times higher than the native j8-CyD, but the affinity for Acridine Red and Rhodamine B was lower than that of the native 8-CyD... 

In two-phase systems, however, where surfactant and water can partition between a fluid and a liquid phase, significant pressure effects occur. These effects were studied for AOT in ethane and propane by means of the absorption probe pyridine N-oxide and a fluorescence probe, ANS (8-anilino-l-naphthalenesulfonic acid) [20]. The UV absorbance of pyridine A-oxide is related to the interior polarity of reverse micelles, whereas the fluorescence behavior of ANS is an indicator of the freedom of motion of water molecules within reverse micelle water pools. In contrast to the blue-shift behavior of pyridine N-oxide, the emission maximum of ANS increases ( red shift ) as polarity and water motion around the molecule increase. At low pressures the interior polarity, degree of water motion, and absorbance intensity are all low for AOT reverse micelles in the fluid phase because only small amounts of surfactant and water are in solution. As pressure increases, polarity, intensity, and water motion all increase rapidly as large amounts of surfactant and water partition to the fluid phase. The data indicate that the surfactant partitions ahead of the water thus there is a constant increase in size and fluidity of the reverse micelle water pools up to the one-phase point. An example of such behavior is shown in Fig. 4 for AOT in propane with a total fVo of 40. The change in the ANS emission maximum suggests a continuous increase in water mobility, which is due to increasing fVo in the propane phase, up to the one-phase point at 200 bar. 

Materials. The synthesis of the p-CD adsorbate is described in references 12, 13, and 14. 6-ferrocenyl-hexanethiol (Fc) was synthesized according to the literature [15] 8-anilino-l-naphthalenesulfonic acid (1,8-ANS), 2-hydroxy-ethanethiol, and octadecanethiol were purchased from Aldrich and used as received. 

Studies have been carried out on the interactions between cyclodextrins and hydrophobic fluorescence probes in water in connection with research on cell adhesion following oligosaccharide/oligosaccharide hydrophobic interactions. While linear oligomers bind 8-anilino-l-naphthalenesulfonate and 6-toluidino-2-naphthalenesuIfonate in 1 1 proportions, the binding constants are smaller than for the corresponding cyclodextrins. ... 

Yamaguchi et al. used poly(NIPAM-co-CD) 4 as a thennosensitive polymer bearing P-CD and 8-anilino-l-naphthalenesulfonic acid (ANS) as a guest molecule (Figure 5.8). The LCST of poly(NIPAM-co-CD) in an aqueous solution was higher... 

Siano and coworkers [14] report the use of 8-anilino-l-naphthalenesulfonic acid (ANS) as a probe of environment polarity in polyacrylamides containing 1 mole percent n-dodecylacrylamide (estimated from feed ratio) in 2 wt% NaCl. ANS, an amphoteric probe is selectively solubilized at the water-hydrophobe interface. The fluorescence emission maximum is shifted from 520 nm, in aqueous environments to approximately 462 nm in hydrophobic environments [18]. Studies of the polymer described above and a homopolymer of acrylamide were accomplished by varying polymer concentration up to 5000 ppm at constant ANS concentration. In Fig. 2.10 a, a plot of the emission wavelength maximum of ANS vs polymer concentration indicated an increasingly hydro-phobic environment with increasing copolymer concentration. Above approximately 1000 ppm, emission wavelength remained constant, indicating that ANS... 

NH4 or Na 8-anilino-l-naphthalenesulfonate, ANS), and a complex probe (pyrene in the presence of MA-dimethylaniline). The I h ratio (the ratio of the fluorescence intensity of the first and third vibronic peaks of pyrene), which indicates the polarity of the probe environment, increases with increasing FHA concentration and the formation of reversed micelles. The formation of reversed micelles is driven by interactions of TCFE, which are lyophobic with the carboxyl group of the surfactant and favorable with the fluorocarbon chain of the surfactant. The aggregation numbers and the amount of water solubilized in the reversed micelles increase with increasing FHA concentration. 

Although the very existence of CMC of surfactant dissolved in nonpolar has been questioned, the UV absorption and fluorescence emission measurements of AOT-solubilized solubilizates, such as tert-butylphenol, pyrene, and hemi-Mg salt of 8-anilino-l-naphthalenesulfonic acid, in isooctane provided evidence for the existence of CMC at different values of R = [H20]/[A0T] (with R < 3) and CMC value increases with increase in R. The values of CMC, obtained at different temperatures, were used to calculate standard enthalpy of micellization (AH j°) from Equation 1.13 and standard entropy of micellization (AS j ) from Equation 1.14, and these respective values vary from 38.07 to 44.81 kJ mol and 184.60 to 202.60 JK moE with increase in temperature from 20 to 45°C. The positive AH j values arise mainly from the dismantling of hydrated ions in the quasi-lattice of AOT interior. 

FIA of anionics without a phase separation may be based on the quenching effect of surfactants on the fluorescence intensity of 8-anilino-l-naphthalenesulfonic acid coupled with bovine serum albumin (83). Another method of avoiding a phase separation is to use a surfactant-selective electrode. Since interference is a problem, the surfactant-selective electrode approach is best coupled with online concentration with a reversed-phase column. Such a system has been demonstrated for trace analysis of sodium dodecylsulfate (84). 

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