Analysis strategies screening

Similar analysis strategies are applied for the peptide mapping of glycoproteins (Medzihradszky et al., 1994 Reinhold et al., 1995 Hancock et al., 1996). Carr and co-workers combined in-source col-lisionally induced dissociation (CID) with LC/MS/MS to identify sites of N- and O-linked glycosylation (Carr et al., 1993). This novel approach uses a series of LC/MS and LC/MS/MS experiments to generate peptide maps and to selectively screen for glycoproteins. 

The laboratory staff, that is, sample preparers, instrument operators, scientists, and synthesists, must join forces to analyze the samples, in order to identify the spiking chemicals with sufficient analytical evidence of acceptable/good quality. The work involves sample preparation, screening and analysis, data evaluation, and eventually making identification an example of an analysis strategy is described in Section 3.4. 

An analysis strategy can generally be outlined in three steps (1) Sample preparation, (2) Screening and Analysis, and (3) Data evaluation and identification examples are described in the following sections. 

Analysis strategies that use on-line ESI-LC/MS approaches provide an integrated format for natural product dereplication by combining traditional fraction collection, sample preparation, and multi-component analysis into a single step. In this way, crude extracts are screened without extensive purification and chemical analysis. Furthermore, less material is required due to the sensitivity of the technique and chromatographic resolution is retained. 

Under contract to the Systems and Strategy Development Division of the OAQPS/EPA, Systems Applications developed and applied modeling methods for the estimation of human exposure and dosage from airborne materials. The model is intended for a screening analysis of the impacts of chemicals under EPA review as potentially hazardous by the definitions of the NESHAPS program. 

Each screening center has medicinal and synthetic chemistry expertise in order to optimize hits identified from HTS campaigns and develop them into chemical probes. Specific capabilities vary, however typical strategies employed include parallel synthesis, computational and informatics analysis, and analytical capabilities such as LC/MS techniques. The structures of novel compounds that are prepared, their synthetic protocols, analytical data and biological data are all available, and samples of final probes developed are deposited into the MLSMR. A Working Group comprised of chemists from each center meets regularly to share information, best practices, and insure optimal use of resources. 

The screening strategy for systematic toxicological analysis (STA) must be very extensive, because several thousands of drugs or pesticides should be considered. In STA the substance(s) present is (are) not known at the start of the analysis. In such a... 

T. (2006) A new strategy of high-speed screening and quantitative structure-activity relationship analysis to evaluate human ATP-binding cassette transporter ABCG2-drug interactions. Journal of Pharmacology... 

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