Propanolol analysis

The utility of hair in the detection of chronic beta-blocker administration was examined by Kintz and Mangin in 8 hypertensive patients. Betaxolol (3 cases, 1.2-2.7 ng/mg), sotalol (2 cases, 4.4-5.3 ng/mg), atenolol (1 case, 0.9 ng/mg), and propanolol (2 cases, 1.6-2.4 ng/mg) were identified. Relative changes in observance of treatment was revealed by sectional hair analysis in a case of betaxolol treatment. 

A NMR study on the formation of diastereoisomeric inclusion complexes between fluorinated amino acid derivatives and a-CD in 10% D2O solution shows that the chemical shifts of the D-amino acid derivatives included by a-CD are upheld from those of their L analogues [77]. The shift difference between the diastereoisomers formed with D and L enantiomers can be used for chiral analysis and optical purity determinations. For example, the interaction of -CD with propanolol hydrochloride produces diastereomeric pairs. The protons of the antipode give NMR signals which differ in chemical shifts in D2O solution at 400 MHz. The intensity of the resonance signals for each diastereoisomer has been used for optical purity determination. By adding racemate to pure (—) isomer, this technique is able to measure optical purity of propanolol hydrochloride in water down to the level of 1%. 

Easterling, D. E., Walle, T., Con-radi, E. C., and Gaffney, T. E. Quantitative Analysis of Naphthoxy-lactic Acid, a Major Metabolite of Propanolol in Plasma in Man... 

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