Analysis of urinary metabolites

Li, C. et al.. Analysis of urinary metabolites of tea catechins by liquid chromatogra-phy/electrospray ionization mass spectrometry, Chem. Res. Toxicol., 14, 702, 2001. 

Metabolic modifications in the chain of reactions involved in tryptophan metabolism were studied by Quagliariello et al. (Q2, Q3) and Piazza and Tancredi (P5) by qualitative analysis of urinary metabolites in 24 patients with viral hepatitis in an acute stage and in 34 subjects at least 2 months after recovery in comparison with normal controls. 

Analysis of In Vivo Fecal Metabolites by LC/MS. Besides the analysis of urinary metabolites, animal metabolism studies of course require characterization of fecal metabolic products. We have found that rapid analysis of fecal extracts can also be accomplished by LC/MS, although more extensive cleanup is required. A general procedure for the extraction and LC/MS analysis of rat fecal metabolites is given in Figure 14. This procedure can easily be completed in an afternoon to provide a preliminary indication of metabolite structures. Based on this information the appropriate derivatives can be prepared for additional characterization, if necessary. 

Barr JR, Young CL, Woolfit AR, et al. Comprehensive quantitative tandem MS analysis of urinary metabolites and albumin adducts following an accidental human exposure to sulfur mustard. In Proceedings ofthe 53rd Conference ofthe American Society of Mass Spectrometry, San Antonio, TX, June 2005. 

P7. Price, J. M., Brown, R. R., and Yess, N., Testing the functional capacity of the tryptophan-niadn pathway in man by analysis of urinary metabolites. Advan. Metab. Disord. 2, 159-225 (1965). 

The process whereby pesticides are registered In Canada Is not unlike that In many other countries. The manufacturer Is required under Federal law to submit, at the time of application for registration, a package of data supporting the safety and efficacy of the product. If after review of these data, the product Is judged to be acceptable. It Is registered and food tolerances are established If required. Over the past 5 years there has been an Increased awareness of the potential health hazards to those Involved In the application of pesticides and those Inadvertently exposed during application (bystanders). To properly analyze these risks, more accurate estimates of exposure are essential. The problems associated with current methods of exposure, the Importance of analysis of urinary metabolites, Che correlation of dermal exposure and urinary metabolites and the determination of percutaneous penetration are discussed. 

The occurrence of 19-hydroxylated PGA, A2, B, and Bj in human seminal plasma [154] suggested the involvement of an human seminal plasma [155] or from seminal plasma that had been subjected to O-methyl-oxime hydrochloride treatment [156]. These results suggested that the previously identified 19-hydroxy-PGA and 19-hydroxy-PGB were artifacts [155]. 19-Hydroxy-PGP and 19-hydroxy-PGp2 were also isolated [157]. Earlier 19- and 20-hydroxyla-tion of PGA, was demonstrated with microsomes of guinea pig liver in the presence of NADPH, while hydroxylation of PGE, occurred only in small amount [158]. On the other hand, analysis of urinary metabolites of PGs revealed the formation of w-oxidized PGs [1,4]. In the presence of NADPH a particulate fraction from lung of... 

Analysis of urinary metabolite of 6-keto-PGF. A Total ion current tracing of peak obtained by HPLC from urine following infusion of radioactive 6-keto-PGFj. 329 scans in the mass range m/z 300-700 were recorded. From the total ion current no identification of a metabolite was possible. The HPLC behaviour of the metabolite made it likely that it was dinor-6keto-PGF. Therefore a reconstructed ion chromatogram for prominent ions of this metabolite i.e. m/z 570, 80 and 350 was obtained from the computer data file. shows which peak of the total ion current represents this metabolite. Instrument HP 5985 A. 

Urine for gas-chromatographic analysis of urinary metabolites None Fresh or frozen 24 h urine (discard the morning urine of the first day but collect the morning urine of the second and final day) Keep frozen (-20 C) until analyzed None, except laboratory error... 

When quercetin-3-rhamnoside was incubated anaerobically with human intestinal bacteria, quercetin, 3,4-hihydroxyphenylacetic acid, and 4-hydroxy-benzoic acid were produced as metabolites [106]. Analysis of urinary metabolites after orally administered mtin labeled with deuterium [(2, 5, 6 -2H]mtin led to the detection of 3-hydroxyphenylacetic acid, 3-methoxy-4-hydroxyphenylacetic... 

Wadman, S.K., Van der Heiden, C., Ketting, D. and Van Sprang, F.J. (1971), Abnormal tyrosine and phenylalanine metabolism in patients with tyrosyluria and phenyketonuria gas-liquid chromatographic analysis of urinary metabolites. Clin. Chim. Acta, 34,277. 

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