Analysis of Genetically Modified Mice

For all genetically modified mice, it is necessary to test first whether homozygous mutant mice are born at Mendelian ratio (embryonic lethality ), are viable, grow normally, are fertile, and have a normal life span. A histochemical analysis should then be carried out of all tissues that express the mutated gene. More specific analyses (e.g., immunofluorescence, electron microscopy or biochemical analysis) of tissues or primary cells derived from the mutant mice depends on the phenotype. 

If the phenotype of the mice is not too severe, then disease models may be applied. Diseases might be induced by the systemic or topic administration of drugs, by surgery, by infections, or by matings with other mice that spontaneously develop diseases. 

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