Anaerobic chromatography

Bruno F, Curini A, Di Corcia A, Fochi I, Nazzari M, Samperi R (2002) Determination of surfactants and some of their metabolites in untreated and anaerobically digested sewage sludge by subcritical water extraction followed by liquid chromatography-mass spectrometry. Environ Sci Technol 36 4156—4161... 

Percentage expresses HPLC recovery of 2-bromo-4,6-dinitroaniline (BDNA). Additional thin layer chromatography measurements indicate anaerobic transformation BDNA... 

The highest degree of purification was achieved with extracellular endopectate lyase from the anaerobic bacterium Clostridium fel-sineum.Mi A 225-fold purified preparation, homogeneous in disc electrophoresis, was obtained by precipitation with ethanol, and chromatography on CM-cellulose and on Sephadex G-200. Its molecular weight (determined by gel chromatography) was 105,000. 

A. Plum, G. Braun and A. Rehorek, Process monitoring of anaerobic azo dye degradation by high-performance hquid chromatography-diode array detection continuously coupled to membrane filtration sampling modules. J. ChmmatogrA, 987 (2003) 395-402. 

The nature of the radioactivity in the water, soil and fish from the carbon-14 DDT experiment was examined by thin-layer chromatography as shown in Figure 5. The radioactivity in the water was very polar in nature and did not migrate appreciably from the origin. About 78% of the radioactivity in the soil was extracted with methanol. The major metabolite in the extractable fraction was DDD which represented 33% of the total radioactivity. The reductive dechlorination of DDT to DDD is a known pathway under anaerobic conditions and has been shown to be due to microbial metabolism (5). Since carbon-14 DDT was incor-... 

To study the effect of the protease treatment cell-free suspension, with or without protease treatment, was subjected to gel-filtration chromatography on Sephadex G-75 and the elution patterns were compared (Fig. 1). In each case, two major peaks were detected by monitoring column fractions with absorbance at 280 nm. Degradation activities on mexacarbate, in the presence of FMN and light under anaerobic condition, were measured for each fraction. It was found that the highest activity was associated with peak II. It is interesting to note that protein (s) associated with peak II were detected with or without protease treatment these will be referred to as natural flavoprotein (B, Fig. 

The sample preparation of endohedral metallofullerenes was done by Shino-hara and details are described in the review article [16]. The soot containing M C2 (M=Sc and La) was produced in direct-current (300-400 A) spark mode under He flow at 50 torr and collected under totally anaerobic conditions. The target fullerenes were separated and isolated from the various hollow fullerenes (C60-C110) and other metallofullerenes by the two-stage high-performance liquid chromatography (HPLC) method by using two complementary types of HPLC columns. The purity of the metallofullerenes used for structure analysis relative to other fullerenes was always more than 99.9%. 

In the acetylene inhibition technique, acetylene is added to a water sample, which inhibits the reduction of N2O to N2 (Sorensen, 1978). The accumulation of N2O is then measured using gas chromatography and an electron capture detector and the denitrification rate is taken to be equal to the total N2O flux. One potential problem is incomplete inhibition of N2O reduction to N2, particularly in the presence of hydrogen sulfide, a compound commonly found under anaerobic conditions. Another potential problem with the technique is that acetylene also inhibits nitrification, a process that often supplies the NOs and N02 substrates for denitrification. To inhibit nitrification is to inhibit denitrification if it is at aU substrate limited (Hynes and Knowles, 1978). 

After anaerobic incubation of reaction mixture at 37°C for 24h, the alkane containing fraction was prepared by chloroform extraction. Chloroform extracts were separated on silica gel 60 thin layer chromatography (TLC) plate with hexane as a developing solvent. Radioactivity was measured by liquid scintillation counter (LS6500, Beckman). 

Materials and Methods section). To deactivate WT hydrogenases, a controlled amount of 02 was added to the anaerobic cell suspension in the sealed vial and incubated under vigorous mixing for 2 min. Anaerobic conditions were then rapidly re-established, and methyl viologen (reduced by addition of sodium dithionite) was added to serve as the electron donor to the hydrogenase. The mixture was then incubated in the dark for 15 min at 30°C, and the reaction was stopped by the addition of trichloroacetic acid. The amount of H2 produced was detected by gas chromatography. 

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