Anaerobic column chromatography

Addition funnel pressure reactor, 201 Adjustable pressure relief valve, 200 Aerial oxidation, 64 Aerobic product transfer, 193 Aerosol pressure vessel, 198 Air-sensitive materials decomposition, 147 HPLC analysis, 24 recovering, 193 synthesis and handling, 34 Alkyne electron density, 287 Alkyne ligand, 282 Alkyne it donor orbitals, 287 Alkyne levels, 285 Ambient pressure flow cell, 238-244 Ammonia synthesis, 182 Anaerobic column chromatography, 17-18/ Anaerobic transfer, 144 Anionic polymerization, 182 Apparatus design philosophy, 117 Arc lamp... 

To study the effect of the protease treatment cell-free suspension, with or without protease treatment, was subjected to gel-filtration chromatography on Sephadex G-75 and the elution patterns were compared (Fig. 1). In each case, two major peaks were detected by monitoring column fractions with absorbance at 280 nm. Degradation activities on mexacarbate, in the presence of FMN and light under anaerobic condition, were measured for each fraction. It was found that the highest activity was associated with peak II. It is interesting to note that protein (s) associated with peak II were detected with or without protease treatment these will be referred to as natural flavoprotein (B, Fig. 

The sample preparation of endohedral metallofullerenes was done by Shino-hara and details are described in the review article [16]. The soot containing M C2 (M=Sc and La) was produced in direct-current (300-400 A) spark mode under He flow at 50 torr and collected under totally anaerobic conditions. The target fullerenes were separated and isolated from the various hollow fullerenes (C60-C110) and other metallofullerenes by the two-stage high-performance liquid chromatography (HPLC) method by using two complementary types of HPLC columns. The purity of the metallofullerenes used for structure analysis relative to other fullerenes was always more than 99.9%. 

Since methloninase has potential applications in both aerobic and anaerobic food systems, a number of experiments were conducted to characterize and evaluate the performance of methloninase under a variety of these conditions. In these studies freeze-dried methloninase preparations (either 0.779 units/mg protein or 2.45 units/mg protein) were employed for generation of methanethiol, and selective flame photometric detection gas chromatography was use for quantifying methanethiol. The column was a 3 m x 4 mm id glass column packed with Carbopak BHT-100, and It was operated at 60°C isothermally for methanethiol analysis. When higher molecular weight sulfur compounds were measured also (e.g.,... 

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