Anabolic agents, extraction

Phylloquinone, menaquinone, and menaphthone have been extracted from serum into supercritical CO2 and separated with SFC, with detection at 250 nm (127). Serum, supported on celite, was transferred to extraction cartridges and extracted with supercritical CO2 at 45°C prior to the analysis of benzodiazepines, anabolic agents, and nonsteroidal anti-inflammatory drugs by HPLC (128). Phenobarbital, butalbital, pentobarbital, and thiopental have been extracted from human serum prior to liquid chromatography-negative-ion electrospray tandem mass spectrometry analysis (129). The sample was extracted with supercritical CO2 at 40°C and 507 bar with static extraction for 5 min followed by dynamic extraction for 30 min. Calibrations graphs were linear for 1-60 pg/mL of each barbiturate and the detection limits were 23, 25, 50, and 225 ng/mL of thiopental, pentobarbital, butalbital, and phenobarbital, respectively. In the determination of the cited analytes in serum spiked at the 10- and 50-pg/mL levels, the recoveries were 94.2-106.9% and the RSDs were 1.14-5.18% (three replicates). 

Huopalahti, R.P. Henion, J.D. Application of supercritical fluid extraction and high performance liquid chromatography/mass spectrometry for the determination of some anabolic agents directly from bovine tissue samples. J.Liq.Chrom.Rel.TechnoL, 1996,19, 69—87... 

Numerous methods has been validated for the determination of anabolic agents (natural and synthetic estrogens, androgens, and gestagens) in edible tissues and in other samples that can be used for food monitoring such as urine, bile, and feces. These methods involve determination of analytes by LC-MS/MS, by GC-MS of suitable derivatives, or by immunological assay, with or without prior LC separation. Typical steps in the extraction, cleanup, and isolation of the analytes are the following ... 

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