Amperometric peroxidase-based sensors

Amperometric peroxidase based H2O2 sensors have been made by using fast reversible redox couples (see Tables I and II). In these, the reducing member of the redox couple (essentially species HA in reaction 1) donates electrons to H2O2 and is oxidized (reaction 2)... 

Kong YT, Imabayashi S, Kano K, Ikeda T, Kakiuchi T (2001) Peroxidase-based amperometric sensor for the determination of total phenols using two-stage peroxidase reactions. Am J Enol Vitic 52 381-385... 

Redox mediators have been used in this case for cathodic detection. An amperometric sensor for choline based on electron transfer between horseradish peroxidase and a redox polymer was described. Amperometric biosensors based on acetylcholinesterase or butyrylcholinesterase were used for the kinetic determination of organophosphate and carbamate pesticides. 

Rajendran V, Csdregi E, Okamoto Y et al. Amperometric peroxide sensor based on horseradish peroxidase and toluidine blue O-acrylamide polymer in carbon paste. Analytica Chimica Acta 1998 373 241-251. 

An amperometric hydrogen peroxide (H2O2] sensor, with a rather low LOD of 6.8 x 10 M, based on bioelectrocatalytic reduction by horseradish peroxidase (HRP] was reported by Luo et al. [41]. HRP was immobilized at a constant potential of 0.7 V and was electrostatically attached on the PANI-MWCNT composite electrode. 

Most studies have focused on amperometric glucose sensors based on GOx or GOx/ peroxidase [168-182]. However, a wide variety of other enzymes, including peroxidase, tyrosinase, choline oxidase, sarcosine oxidase, cholesterol oxidase, xanthine oxidase, glutamate oxidase, glutamate dehydrogenase, nitrate reductase, cholinesterase, cholesterol esterase, uricase, urease, creatinase, and creatininase, have also been employed for bioelectrosensor construction [183-203]. Representative examples are outlined in Table 2. 

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