Aminoglycosides analysis

The separation mechanism in the LC analysis of aminoglycosides is usually highly dependent on the applied derivatization technique, either precolumn or postcolumn. This is due to the fact that a prerequisite of aminoglycosides analysis is most often suitable derivatization to produce fluorescent derivatives the presence of primary amine groups in most of the aminoglycoside antibiotics enables a number of derivatives to be readily formed. 

In Table 4, the different methods of aminoglycoside analysis are summarized. 

Obtain serum drug levels for aminoglycosides and/or vancomycin and perform pharmacokinetic analysis. Adjust the dose, if needed, according to the parameters in Table 13-2. Obtain follow-up trough levels at weekly intervals or sooner if renal function is unstable. Follow serum creatinine levels if renal function is unstable. Hearing tests may be scheduled yearly or per patient preference. 

Hooker KD, DiPiro JT, Wynn JJ. Aminoglycoside combinations versus single [1-lac la ms for penetrating abdominal trauma A meta-analysis. J Trauma 1991 31 1155-1160. 

Flurer, C. L. (1995). The analysis of aminoglycoside antibiotics by capillary electrophoresis. /. Fharm. Biomed. Anal. 13, 809—816. 

Vicens, Q. Westhof, E., Molecular recognition of aminoglycoside antibiotics by ribosomal RNA and resistance enzymes An analysis of x-ray crystal stmctures. 

Although a number of chromatographic methods have been reported for determinations of aminoglycoside antibiotics in blood serum and urine, the application of chromatographic methods to residue analysis has been very limited. Shaikh et al (93) recently described an HPLC method for neomycin in animal tissue, and Lachatre et al (94) described a method for nine aminoglycosides in plasma, urine, and renal cortex tissue. Both procedures use post column derivatization with a-pthalaldehyde and fluorescence detection. 

Van Lent-Evers, N.A., Mathot, R.A., Geus, W.P., van Hour, B.A., and Vinks, A.A. (1999) Impact of goal-oriented and model-based clinical pharmacokinetic dosing of aminoglycosides on clinical outcome a cost-effectiveness analysis. Ther Drug Monit 21 63-73. 

Contopoulos-Ioannidis DG et al Extended-interval aminoglycoside administration for children A meta-analysis. Pediatrics... 

Paul M et al Beta lactam monotherapy versus beta lactam-aminoglycoside combination therapy for sepsis in immunocompetent patients Systematic review and meta-analysis of randomised trials. BM3 2004 328 668. [PMID 14996699]... 

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