Aminoglycoside-modifying Enzyme Inhibitors Targeting Ribosomal RNA

Aminoglycoside-modifying Enzyme Inhibitors Targeting Ribosomal RNA 

Utility Treatment of Drug Resistant Bacterial Disorders 

Preparation of 5-ethylcarboxyl-l, 3,2, 6 -tetraazido-6,3,4 -tri-0-benzyl-neamine diamine dimer 

Protected neamine dimers were prepared using a Quest 210 parallel synthesizer. 5-Ethylcarboxyl-1,3,2, 6 -tetraazido-6,3, 4 -tri-6 -benzylneamine (0.0826 mmol/tube was dissolved in 1.5ml/tube containing CH2C12 distributed to each tube, which was then treated with MP-carbodiimide resin (1.15mmol/g) followed by 1,12-dodecylamine (0.0413 mmol/tube). Solutions were agitated 16 hours, filtered, concentrated, and dimers isolated as colorless foams. 

Antimicrobial testing was performed using the Kirby-Bauer Disk assay as described by Hendrix (1) and results summarized in Table 3. 

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I. Aminoglycoside-modifying Enzyme Inhibitors Targeting Ribosomal RNA... 

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