Conjugated amino group

In order to investigate dendrimers of a different nature [230], Bradley described the synthesis and transfection efficiency of polyamidourea dendrimers synthesised from isocyanate-containing AB3 monomers [231-234]. The use of this kind of tris-branched building block was addressed to enhance dendrimer synthesis by replacement of the 1,4-addition step typical of PAMAM synthesis and to a rapid increase in terminal functionality. The dendritic structures were synthesised using a divergent, microwave-assisted, solid-phase approach with the dendrimers assembled on polystyrene resin via an acid labile linker (see Fig. 26). In particular, a G3.0 polyamidourea bis-dendron with the peripheral amino groups conjugated to L-lysine residues demonstrated remarkable transfection abilities [234],... 

When the nitro group is para to the amino group, conjugation of the amino group with the nitro group can also occur. p-Nitroaniline is thus even less basic than m-nitroaniline. 

They may also be detoxified by conjugation with glutathione [115]. For instance dehydroretronecine is converted in rat liver to 7-glutathionyldehydroretronecine. [116]. Dehydromonocrotaline, produced from monocrotaline is believed to be the active toxic metabolite. It reacts at its C-9 or C-7 position to alkylate the N-3 position of several purine nucleosides or various amino groups conjugation with glutathione. Apparent... 

The most used EIA reagents conjugate a fluotophote such as fluorescein-isothiocyanate (EITC) or thodarnine—isothiocyanate to antibody (or antigen) free amino groups. Examples of other commonly used fluotophotes for EIA and their spectral characteristics ate presented in Table 3. EIA assays ate available in sandwich and competitive formats similar to EIAs. Unlike EIA kits which can be used directly with visual color deterrnination, EIAs require a fluorometer, and thus ate primarily laboratory-based. 

Oxidative Couplings of Heterocyclic Hydrazones. This method has opened the way to the preparation of azo derivatives of diazo compounds unobtainable by other means, ie, heterocycHc compounds ia which the diazotizable amino group is conjugated with the heterocycHc nitrogen atom as ia 2- and 4-amiQopyridine, compounds which do not normally yield stable diazonium salts (38). The reaction occurs as illustrated by equation 7 for the iateraction of (A/-methylcarbostyryl)hydrazone [28219-37-6] and dimethyl aniline the overall process is oxidation. 

Conjugation of the amino group of an aiylfflnine with a second aiomatic ring, then a thud, reduces its basicity even further. Diphenylamine is 6300 times less basic than aniline, whereas triphenylamine is scarcely a base at all, being estimated as 10 ° times less basic than aniline and 10 " times less basic than ammonia. 

Attachment of a basic amino group to the side chain leads to a compound with antiparkinsonian activity. Alkylation of the carbanion from phenylacetonitrile with 2-chlorotriethylamine affords the product, 36. Conjugate addition of the anion from this to acrylonitrile gives the glutarodinitrile (37). Partial hydrolysis of this in a mixture of sulfuric and acetic acid leads to phenglutarimide (38). ... 

Conjugated cyclohexenones [6] have also been easily prepared by combining the cycloaddition of dimethylaminobutadiene 4 and several cyclic and acyclic dienophiles followed by the elimination of the amino group from the cycloadducts under acidic conditions. Scheme 2.3 summarizes some of these results. 

Haptens with an amino group. Amine groups in haptens, carrier proteins or both can be modified for conjugation through homo- or heterobifunctional crosslinkers such as acid anhydrides (e.g., succinic anhydride), diacid chlorides (e.g.. 

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