Amino acids applications

T Hayashi, H Tsuchiya, H Naruse. Reversed-phase ion-pair chromatography of amino acids. Application to the determination of amino acids in plasma samples and dried blood on filter papers. J Chromatogr Biomed Appl 274 318-324, 1983. 

One of the first applications of HPLC in the clinical field was the quantitation of theophylline in asthmatic infants. This highly accurate measurement was an important test because of the very low amount of sample required and the accuracy of the determination (see Fig. 1-10). More recent clinically related HPLC separations include drugs and drug metabolites, neurochemicals and their metabolites, histamines, thyroid hormones, and enkephalins. The earliest bioresearch applications of HPLC included the determination of peptides, proteins, and amino acids. Application of HPLC to the analysis of these compounds remains important, as indicated by the rapid growth in references (Fig. 1-11). Bioresearch remains one of the most rapidly expanding growth areas of LC. 

Unlike mammalian cells, which cannot synthesize all amino acids, SILAC is not well suited to studying the protein/peptide levels of microorganisms, most of which are prototrophic for all amino acids. Application of SILAC in such cases is mostly restricted to auxotrophic strains, which renders the technique ineffective for the proteome analysis of many commercially important microbes. Native SILAC (nSILAC), a recently developed modification of... 

PROTEIN ENGINEERING WITH NONCODED AMINO ACIDS APPLICATIONS TO HIRUDIN... 

Vincenzo De Filippis, Department of Pharmaceutical Sciences, University of Padova, Padova, Italy, Protein Engineering with Noncoded Amino Acids Applications to Hirudin... 

Amide- and Urea-Based Anion Receptors, p. 31 Amino Acids, Applications, p. 42... 

Amino Acids Applications, p. 42 Anion-Directed Assembly, p. 51 Anticrowns, p. 68... 

Amide- and Urea-Based Anion Receptors, p. 51 Amino Acids Applications, p. 42 Chiral Guest Recognition, p. 236 Deoxycholic, Cholic, and Apocholic Acids, p. 441 Fluorescence Sensing of Anions, p. 566 Guanidinium-Based Anion Receptors, p. 615 Hydrogen Bonding, p. 658 lonophores, p. 760... 

Reactions of ammonia and amines with C = C double and C = C triple bonds are interesting because they allow the preparation of many valuable compounds [see Section 6.A.11.C and reactions (13.91) and (13.92)]. For example, the addition of ammonia to unsaturated acids would allow the preparation of amino acids. Application of catalysts with asymmetric ligands for this reaction could lead to synthesis of optically active amino acids, which already exist in nature. 

M.H. Joseph and P. Davies, Electrochemical activity of o-phthalaldehyde-mercaptoethanol derivatives of amino acids. Application to high-performance liquid chromatographic determination of amino acids in plasma and other biological materials, J. Chromatogr., 1983, 277, 125-136. 

Nagata T. and Kirchman D.L. 1990. Filtration-induced release of dissolved free amino acids application to cultures of marine protozoa. Mar. Ecol. Prog. Ser. 68 1-5. 

Igumenova and McDermott have reported an analysis of carbon line shapes in high resolution solid-state NMR spectra of uniformly C-enriched amino acids.Application of a /-decoupling protocol during the carbon chemical shift evolution period allowed to separate the contribution of the second-order dipolar shift from that of the /-coupling interactions to carbon line... 

Yang 1997 Yang, L. and Chiu, K., Solid Phase Synthesis of Fmoc N-Methyl Amino Acids Application of the Fukuyama Amine Synthesis, Tetrahedron Lett., 38 (1997) 7307-7310. 

Han Z, Yamaguchi Y, Kitamura M, Maruoka K. Convenient preparation of highly active phase-transfer catalyst for catalytic asymmetric synthesis of a-alkyl- and a,a-dialkyl-a-amino acids application to the short asymmetric synthesis of BlRT-377. Tetrahedron Lett. 2005 46(49) 8555-8558. 

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