Amino acid analysis carbamate derivatization

SA Cohen, C Van Wandelen. Amino acid analysis of unusual and complex samples based on 6-aminoquinolyl-V-hydroxysuccinimidyl carbamate derivatization. Techniques in Protein Chemistry VIII 185-196, 1997. 

Amino Acid Analysis of Unusual and Complex Samples Based on 6-aminoquinolyl-N-hydroxysuccinimidyl Carbamate Derivatization... 

The analysis of cell culture media and supernatants, as well as non-standard protein hydrolysates such as collagens and glycoproteins, has created the demand for techniques that accurately quantify additional amino acids not normally found in hydrolyzed samples, Methods of amino acid analysis (AAA) based on precolumn derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) have previously been shown to quantify hydrolyzed samples with a high degree of accuracy (1,2). The AQC-based method has also been shown to derivatize effectively in the presence of salts and lipids (3). Taking into account the above strengths, the excellent stability of the derivatives, and the unique fluorescence properties that allow for direct injection of the reaction mixture without cleanup, the AQC methodology represents an ideal choice for the analysis of complex samples. 

A Note on Post-Column Reaction Techniques A post-column reaction unit is an online derivatization system that supplies reagents to the column eluent into a heated chamber to convert the analytes into more chromophoric forms for higher sensitivity detection. Some common applications of post-column reaction systems are amino acid analysis using ninhydrin (with visible detection), and carbamate pesticide analysis using o-phthaldehyde (with fluorescence detection).]... 

Cohen SA (2003) Amino acid analysis using pre-column derivatization with 6-aminoquinolyl-N-hydroxysuccini-midyl carbamate. Analysis of hydrolyzed proteins and electrohlotted samples. Methods in Molecular Biology 211 143-154. 

SA Cohen, DP Michaud. Synthesis of a fluorescent derivatizing reagent, 6-aminoquinolyl-A -hydroxysuccinimidyl carbamate, and its application for the analysis of hydrolysate amino acids via high-performance liquid chromatography. Anal Biochem 211 279-287, 1993. 

SA Cohen, KM DeAntonis. Applications of amino acid derivatization with 6-aminoquinolyl-Al-hydroxysuccinimidyl carbamate. Analysis of feed grains, intravenous solutions and glycoproteins. J Chromatogr A 661 25-34, 1994. 

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