Alloantigen presenting cells

Previous studies on the effects of N=0 production on the alloimmune response utilized bulk populations of responder and stimulator cells. In order to more clearly define the circumstances that induce -N=0 synthesis in allogeneic macrophage-lymphocyte cocultures, mouse splenocyte populations were depleted of accessory cells (>90% Thy 1.2+) and cultured with mitomycin-C-treated macrophage cell lines, as the alloantigen presenting cells. The P388D1 (H2 ) and RAW 264.7 (H2 ) macrophage lines were selected because... 

Cooper, K. D. et al., Effects of UVR on human epidermal cell alloantigen presentation Initial depression of Langerhans cell dependent function is followed by the appearance of T6-DR+ cells that enhance epidermal alloantigen presentation, J. Immunol. 134,129-137, 1985. 

Indirect pathway. Recipient APCs migrate into the graft and phagocytize alloantigens. The donor MHC molecules then are expressed on the cell surfaces of the recipient s APCs and presented to recipient T cells in the lymph nodes. 

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