Allele-specific differential expression

The analysis of gene differential expression is complicated by the potentially subtle differences associated with alterations in a single allele as well as by variations between individuals that arise from environmental or physiological factors. To circumvent these analytic problems, a method, named allele-specific differential expression analysis, was developed to compare the relative expression levels of two alleles of the same gene within the same cellular sample. The studies of allele-specific expression revealed that differential expression is relatively common in the human population. 

Many other applications have been carried out using semi-quantitative primer extension based MALDI-TOF MS analysis with unknown analyte concentrations. One example involves the quantification of differential allele-specific expression. Medelian inheritance of allele-specific expression has been shown in humans (40). Allele-specific expression is of great importance for the identification of genetic components, which lead to population... 

Assaying Differential Ribosomal RNA Gene Expression with Allele-Specific Probes... 

Light chains expressing specificities b4, b5, or b6 also have distinct C-terminal sequences (35,36a). (These are shown in Table 9.2 together with C-terminal sequences of human and mouse L chains, included for the sake of comparison.) In addition, Frangione and Lamm have isolated two small Internal peptides, adjacent to half-cystine residues, with amino acid sequences that differentiate the b4 and b5 alleles (36). The same amino acids that are involved in the sequence differences identified so far were also implicated by data on overall amino acid composition. 

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