Alkyl groups, bonded, HPLC development

Adsorption surface, SFC, solvent Interactions, 1>>9 Air monitors. Industrial chromatography, 200 Alditol acetates, chromatogram, 32f Alkyl groups, bonded, HPLC development, 82,83 Amides, enantiomeric, separation, >l3,>l>lf Amine compounds, RPLC, 8>l Amino acids HPLC, 86,87f Ion exchange, 81 ligand exchange, >>... 

Bonded alkyl functional groups, HPLC development, 83... 

Over 500 HPLC packings have been described in the Hterature. Nevertheless, as the result of years of development, only a limited number of types of stationary phases remain on the market. Most of the conventional HPLC separations today are performed using monodisperse silica gel 3 or 5 Xm microbeads, especially those grafted with C4, C8, or C18 alkyl chains, as well as with cyano-propyl or amino-propyl groups. The last two bonded silicas and bare silica are used in normal phase (NP) HPLC, where the mobile phase (usually hexane with small amounts of isopropyl alcohol) is less polar than the stationary phase. Even more popular is the reversed phase (RP) mode, which uses polar eluents (mosdy water or methanol with such additives as acetonitrile, methanol, or tetrahydrofuran (THE)) in combination with nonpolar alkyl-bonded stationary phases. 

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