Diamine-modified

The diamine-modified DNA may be isolated from excess reactants by ethanol precipitation according to steps 4-7 of the protocol described previously for nick translation (Section 1, this chapter). Alternatively, dialysis or gel filtration may be done to remove excess reactants. 

Reaction of NHS-LC-Biotin with Diamine-Modified DNA Probes... 

Alkaline Phosphatase Conjugation to Diamine-Modified DNA Using DSS... 

Figure 27.15 The homobifunctional crosslinker DSS may be used to conjugate an enzyme to a 5 -diamine-modified oligonucleotide. The NHS ester groups on DSS react with the amines to form amide bonds.

Conjugation of Amine-Reactive Fluorescent Probes to Diamine-Modified DNA... 

It has been shown that the thus rubber bound antioxidant can reduce the amount of plasticizer required for compounding and that the ozone resistance of a vulcanizate containing a p-phenylene diamine modified PIB is superior to that of other vulcanizates (71). 

Current efforts are directed towards (i) mechanistic issues to rationalize the effectiveness of the diamine-modified system in protic solvent in comparison to Zn-hydride catalyst, and (ii) the development of an efficient enantioselective version of this system with chiral diamines. 

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