Alkaline lipase

Animal fat cmd vegetable oil hydrolysis during laundry wash Alkaline lipase... 

Lipolysis in mould-ripened varieties is due primarily to the lipases of Penicillium roqueforti or P. camemberti, which secrete potent extracellular lipases. Penicillium lipases are well characterized (see Kinsella and Hwang, 1976 Gripon, 1987,1993). P. camemberti appears to excrete only one lipase which is optimally active at ca. pH 9.0 and at ca. 35°C. P. roqueforti excretes two lipases, one with a pH optimum at 7.5 to 8.0 (or perhaps 9.0 to 9.5), the other at pH 6.0 to 6.5 The acid and alkaline lipases exhibit different specificities. Lipases of Geotrichum candidum have been studied by Side-bottom et al. (1991) and Charlton et al. (1992). This organism produces two lipases of different substrate specificities. 

Rathi P, Bradoo S, Saxena RK et al. (2001) A novel alkaline lipase from Burkholderia cepacia for detergent formulation. Proc Biochem 37 187-192 Reetz MT, Wiesenhofer W (2004) Liquid poly(ethylene glycol) and supercritical carbon dioxide as a biphasic solvent system for lipase-catalyzed esterification. Chem Commun 2750-2751 Rehdorf J, Kirschner A, Bornscheuer UT (2007) Cloning, expression and characterization of a Baeyer-Villiger monooxygenase from Pseudomonas putida KT2440. Biotechnol Lett 29(9) 1393-1398... 

Hong MC, Chang MC (1998) Purification and characterization of an alkaline lipase from a newly isolated Acinetobacter mdioresistem CMC-1. Biotechnol Lett 20 1027-1029 Hong Y, Wang TW, Hudak KA et al. (2000) An ethylene-induced cDNA encoding a lipase expressed at the onset of senescence. Plant Biol 97 8717-8722 Houde A, Kademi A, Leblanc D (2004) Lipases and their industrial applications an overview. Appl Biochem Biotechnol 118(1-3) 155-170... 

Wang Y, Srivastava KC, Shen GJ et al. (1995) Thermostable alkaline lipase form a newly isolated Bacillus strain, A30-1 (ATCC 5384). J Eerment Bioeng 79 433-438... 

Chen, S., C. Cheng, and T. Chen. 1998. Prodnction of an Alkaline Lipase hy Acinetobacter Radioresistens. Journal of Fermentation and Bioengineering 86 (3) 308-312. 

Kojima, Y., M. Yokoe, and T. Mase. 1994. Purification and Characterization of an Alkaline Lipase from Pseudomonas Fluorescens Akl02. Bioscience, Biotechnology, and Agrochemistry 49 (9) 1564-1568. 

Wang, Y., K. C. Srivastava, G. Shen, and H. Y. Wang. 1995. Thermostable Alkaline Lipase from a Newly Isolated Thermophilic Bacillus, Strain A30-1 (ATCC 53841). Journal of Fermentation and Bioengineering 79 (5) 433—438. 

The secretion machinery of Pseudomonas alcaligenes, the producer of Lipomax , an alkaline lipase for detergent compositions. The XcpQ protein forms a multimeric ring in the outer membrane. The Xcp T, U, V, and W proteins are thought to be involved in the gating of the pore, and proteins P, X, Y, and Z form a connection between the outer membrane pore and the inner membrane. XcpA, S, and P play a role in the processing of other Xcp proteins. Sec is inner membrane translocase. Ch is the periplasmic chaperone... 

Rathi P, Saxena RK, Gupta R (2001) A novel alkaline lipase from Burkholderia cepacia for detergent formulation. Process Biochem 37 187-192 Rawlings ND, Barrett AJ (2004) Introduction metallopeptidases and their clans. In Barrett AJ, Rawlings ND, Woessner JE (eds) Handbook of proteolytic enzymes, 2nd edn. Elsevier, London, pp 231-268 Rawlings ND, Bateman A (2009) Pepsin homologues in bacteria. BMC genomics 10 437 46... 

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