Albumin attenuated total reflection

In the present studies, a Fourier-transform infra-red spectrometer in attenuated total reflectance mode (FT-IR-ATR) was used to characterize the albuminated polymer membrane surface. FT-IR-ATR is a powerful surface analysis technique in which a spectrum of only a few micron thick surface layer is obtained. As albumin or enzymes were attached only on the surfaces of polypropylene, FT-IR-ATR technique was ideally suited for the their analysis. 

J.W. Hail, A. Pollard Near-infrared spectroscopic determination of serum total proteins, albumin, globulins and urea. Clin. Biochem. 26, 483 (1993) Y. Mendelson, A.C. Clermont, R.A. Peura, B.-C. Lin Blood glucose measurement by multiple attenuated total reflection and infrared absorption spectroscopy. lEEB Tkans. Biomed. Eng. 37, 458 (1990)... 

Murayama, K., Wu, Y, Czarnik-Matusewicz, B. and Ozaki, Y. (2001) Two-dimensional/attenuated total reflection infrared correlation spectroscopy studies on secondary structural changes in human serum albumin in aqueous solutions pH-dependent structural changes in the secondary structures and in the hydrogen bondings of side chains. J. Phys. Chem. B, 105,4763-4769. 

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