Agar gel

The evolution of nitrogen aids in removing dissolved air. A salt bridge (4 mm tube) attached to the saturated calomel electrode is filled with 3 per cent agar gel saturated with potassium chloride and its tip is placed within 1 mm of the mercury cathode when the mercury is not being stirred this ensures that the tip trails in the mercury surface when the latter is stirred. It is essential that the mercury-solution interface (not merely the solution) be vigorously stirred, and for this purpose the propeller blades of the glass stirrer are partially immersed in the mercury. 

FIG. 9 Polyacrylamide and agarose size distribution, (a) Agar gels, (b) polyacrylamide gels, and (c) agarose gels. (Reprinted with permission from Ref. 79, Copyright 1995, Academic Press.)... 

CK isoenzymes have been separated by electrophoresis on cellulose acetate, agar gel, agarose and polyacrylamide gel. 

This sheet micro flow reactor (Figure 4.32) was used for investigating spatially two-dimensional effects in reaction media using agar gel induced by electric fields [68]. This device utilizes an adapted Petri dish which comprises a rectangular channel... 

E Schantz, M Lauffer. Diffusion measurements in agar gel. Biochemistry 1 658, 1962. 

Rps. palustris 420L, entrapped into agar gel, malate,glutamate, yeast extract, over 30 days of 1.2 40 0.0178 44 Vincenzini et al., 1981... 

Hirschfeldt (H8) has suggested that the small differences in migration rate of the different HpHb complexes in agar gel at pH 8.6 with a Ca++-containing barbiturate buffer can be used for ascertaining the Hp type. In our opinion the results obtained by this simplified procedure are less reliable than those obtained by the somewhat more complicated starch gel electrophoretic method. 

H8. Hirschfeld, J., A simple method of determining haptoglobin groups in human sera by means of agar-gel electrophoresis. Acta Pathol. Microbiol. Scand. AT, 169 (1959). 

Fig. 6. MR wave image of acoustic refraction. Shear waves generated in the upper part of an agar gel phantom (horizontal motion) propagate vertically in the stiff part of the phantom (/i 50 kPa cT 7.5 cm/s) and are refracted by the oblique lower part of soft gel (fi 15 kPa cT 4 cm/s). Note the marked reduction of wavelength in the softer medium. From Ref. 23, reprinted by permission of Wiley-Liss, Inc., a subsidiary of John Wiley Sons, Inc.

Another analysis method was based on the local wave vector estimation (LFE) approach applied on a field of coupled harmonic oscillators.39 Propagating media were assumed to be homogeneous and incompressible. MRE images of an agar gel with two different stiffnesses excited at 200 Hz were successfully simulated and compared very well to the experimental data. Shear stiffnesses of 19.5 and 1.2 kPa were found for the two parts of the gel. LFE-derived wave patterns in two dimensions were also calculated on a simulated brain phantom bearing a tumour-like zone and virtually excited at 100-400 Hz. Shear-stiffnesses ranging from 5.8 to 16 kPa were assumed. The tumour was better detected from the reconstructed elasticity images for an input excitation frequency of 0.4 kHz. 

Agar gel containing 10% (v/v) of a polyclonal antibody to human albumin. 

Steere, R. E., and G. K. Ackers Restricted diffusion chromatography through calibrated columns of granulated agar gel a simple method for particle-size determination. Nature /9d, 475 76 (1962). 

Fig. 13. The nuclear magnetic spin-lattice relaxation rate for water protons as a function of magnetic field strength reported as the proton Larmor frequency at 298 K for 5% suspensions of the particulate stabilized in a 0.5% agar gel presented as the difference plot (A) Zeolite 3A (B) Zeolite 13X (C) Zeolite NaY (D) kaolin with 7 s added to each point to offset the data presentation (E) Cancrinite with 9 s added to each point to offset the data presentation and (F) 0.5% agar gel profile with 10 s added to each point. The solid lines are fits to a power law (68).

High voltage electrophoresis (HVE) in agar gel with detection by bioautography has been used with considerable success in some laboratories for identification of residues (1-6). This procedure has the advantage that all antibiotic substances detectable by bioautography can be classified on the basis of electrophoretic mobility. Further testing may be required for quantification and to... 

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