Size agarose

Zhou, Q.Z., Wang, L.Y., Ma, G.H., and Su, Z.G. 2007. Preparation of uniform-sized agarose beads by micro-porous membrane emulsification technique. J. Colloid Interface Sci. 311 118-127. 

An example of a size-exclusion chromatogram is given in Figure 7 for both a bench-scale (23.5 mL column) separation and a large-scale (86,000 mL column) mn. The stationary phase is Sepharose CL-6B, a cross-linked agarose with a nominal molecular weight range of 5000-2 x 10 (see Fig. 6) (31). 

Bead Composition (% agarose) Size exclusion pore dimension° (nm) Fractionation range Maximum Maximum operating linear velocity (cm/hr) ... 

Superose gel material of Pharmacia Biotech is a highly epichloro-hydrine cross-linked agarose matrix that has a pH range of 3-12 (short term 1-14). Hydrophilic interactions may be noticeable for lipids, peptides, and small aromatic compounds, but such interactions might even improve resolution. Superose medium is available in two different porosities Superose 6 HR 10/ 30 (bead size 13 2 /um maximum pressure 1.5 MPa) and Superose 12 HR 10/30 (bead size 10 2 /um maximum pressure 3.0 MPa). 

Generally, electrophoresis is carried out not in free solution but in a porous support matrix such as polyacrylamide or agarose, which retards the movement of molecules according to their dimensions relative to the size of the pores in the matrix. 

FIG. 9 Polyacrylamide and agarose size distribution, (a) Agar gels, (b) polyacrylamide gels, and (c) agarose gels. (Reprinted with permission from Ref. 79, Copyright 1995, Academic Press.)... 

Chiari, M D Alesio, L Consonni, R Righetti, PG, New Types of Large-Pore Polyacrylamide-Agarose Mixed-Bed Matrices for DNA Electrophoresis Pore Size Estimation from Ferguson Plots of DNA Eragments, Electrophoresis 16, 1337, 1995. 

Agarose gel electrophoresis can be used to determine whether the PCR amplicon is the expected size. The density of the gel should be chosen to ensure resolution of... 

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