Aflatoxins from corn, peanuts peanut butter

Extraction of Aflatoxins from Corn, Peanuts, and Peanut Butter... 

Elute column with 6 mL of chloroform/acetone (9 1). Bakerbond Application Note FF-013 Extraction of Aflatoxins from Corn, Peanuts, and Peanut Butter (See Suggested Reading, Chapter 1). 

The extraction of aflatoxins (Fig. 5.9) from corn, peanuts, and peanut butter involves the dissolution of the aflatoxins in an aqueous methanol extract, followed by extraction with chloroform and clean-up on a silica-gel column. The extraction is carried out with 50 g of food material and 200 mL of methanol/water (85 15) for 30 min. Filter 40 mL of the extract and collect the filtrate. Add 40 mL of 10% sodium chloride to salt out the aflatoxin and extract with 2 X 25 mL of chloroform. Evaporate to dryness and redissolve with 3 mL of methylene chloride. Sample is ready for addition to the silica-gel column that contains 0.5 g of packing material. The mechanism of sorption involves hydrogen bonding between the oxygen atoms of the aflatoxin and the silica gel. Sorption is from methylene chloride and elution is carried out with chloroform/acetone (9 1). 

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