Affinity chromatography separation

C. King The Use of Coenzymes in Biochemical Reactors. - C. Wandrey, E Flaschel Process Development and Economical Aspects in Enzyme Engineering Acylase-L-Methionine System. - D.J. Graves, Yun-Tai Wu The Rational Design of Affinity Chromatography Separation Processes. 

P.C. Wankat, Theory of affinity chromatography separations, Anal. Chem., 46(1974)1400. 

Affinity chromatography separation effected by affinity of solute molecules for a bio-specific stationary phase consisting of complex organic molecules bonded to an inert support material, e.g. separation of proteins on a bonded antibody stationary phase. The technique is really selective filtration rather than chromatography. 

Gel-filtration chromatography separates proteins based on size. Ion-exchange chromatography separates proteins based on net charge. Affinity chromatography separates proteins based on their affinity for specific ligands. To purify a protein, many techniques are used and often several different chromatography steps are used. 

There are many advantages in using a monolith for affinity chromatography separations ... 

ATIIl has been also used as a model protein to test a novel affinity chromatographic system capillary affinity chromatography. Separation quality has been foimd equivalent to that observed with classical affinity chromatography, whereas the necessary protein amount is strongly reduced to the nanogram level. 

Bulk cellulose is a poor affinity-chromatographic matrix its principal advantage is its low cost. This is rarely an overriding consideration since the enzymes to be purified are usually expensive. Cellulose was employed in 1953 in one of the earliest affinity-chromatography separations, where tyrosinase was purified on a column of aromatic cellulose ethers (27). Currently, cellulose (28) or related polysaccharides (29) are used in a number of applications as a bioselective adsorbent including DNA-cellulose for the purification of DNA-binding proteins. 

FIGURE 4.2 Typical affinity chromatography separation. (See insert for color representation of the figure.)... 

Purification of neuraminidase by affinity chromatography separation of two forms of neuraminidase 154... 

Removal of polysaccharide contaminants from preparations of DNA by affinity chromatography Separation of membrane glycoproteins from rat-brain synaptic vesicles by affinity chromatography Studies of the interactions of the carbohydrate-binding sites on concanavalin A with adipocyte receptors... 

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