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Affinity chromatography of immunoglobulin

Affinity Chromatography of Immunoglobulins on immobiiized Antibodies (Immunoaffinity Chromatography, iAC)... [Pg.117]

Jeffrey, A.M., Zopf, D.A., and Ginsburg, V. (1975) Affinity chromatography of carbohydrate-specific immunoglobulins coupling of oligosaccharides to sepharose. Biocbem. Biopbys. Res. Comm. 62, 608-613. [Pg.1079]

Affinity chromatography of surface glycoproteins and immunoglobulin E receptors from rat basophilic leukaemia cells Affinity chromatography of membrane immunoglobulin M and murine lymphocyte immunoglobulin study of their affinities for the lectin... [Pg.603]

Affinity chromatography of glycopeptides obtained 263 by pronase digestion of immunoglobulin G from a patient with multiple myeloma... [Pg.756]

Purification of a cyclic nucleotide phosphodiesterase Affinity chromatography of carbohydrate-specific immunoglobulins Purification of chicken pepsin... [Pg.457]

Antibodies.— Antibodies to the milk oligosaccharide (40), viz. anti-Oacto- -difucohexaose) antibody, have been complexed with succinylated haemocyanin as part of a study of the affinity chromatography of carbohydrate-specific immunoglobulins. ... [Pg.444]

Hjelm H, Hjelm K, Sjoquist J. Protein A from Staphylococcus aureus. Its isolation by affinity chromatography and its use as an immunosorbent for isolation of immunoglobulins. FEBS Lett 1972 28 73-76. [Pg.110]

Affinity chromatography and related techniques (e.g., thiol chromatography and IMAC) are widely used for preparative isolation because they enable a single protein or class of proteins to be selectively purified from very complex mixtures. They may be occasionally used as analytical tools. For example, protein A affinity chromatography has been used for quantitative analysis of immunoglobulins in ascites fluid.45 Information about surface-accessible histidine and phosphate groups may be obtained using IMAC. [Pg.60]

Other common impurities, such as immunoglobulins and protein A, result from the immunoaffinity purification of recombinant proteins or MAbs.16 If affinity chromatography is used to purify an antigen, then an ELISA can be used to detect contaminating levels of MAbs leached from the column. An assay for the antibody needs to detect the antibody in the presence and absence of its specific antigen. [Pg.291]

Table 4.8. Binding affinities of immunoglobulin subclasses from different species to immobilized Protein A, Protein G, Protein L, and thiophilic chromatography supports... Table 4.8. Binding affinities of immunoglobulin subclasses from different species to immobilized Protein A, Protein G, Protein L, and thiophilic chromatography supports...
Figure 26-16 shows the isolation of the protein immunoglobulin G (IgG) by affinity chromatography on a column containing covalently bound protein A. Protein A binds to... [Pg.602]

Protamine sulfate has a low affinity for IgM and is only recommended for producing small amounts of immunoglobulin. Some form of enrichment procedure before chromatography is advised. [Pg.117]


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See also in sourсe #XX -- [ Pg.2 , Pg.291 , Pg.292 , Pg.293 , Pg.294 , Pg.295 ]




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Affinity chromatography

Of immunoglobulins

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