Affinity chromatography design

Receptor affinity chromatography, 3 847 Receptor blockers, 5 158 Receptor design, principles of, 76 769-775 Receptor purity, in microarray fabrication, 76 383... 

The best column design for GPC as well as for affinity chromatography is a column with adaptors at both ends (Fig. 3.3C). This column type guarantees even surfaces of the gel bed, symmetrical application of sample, and regular flow of the eluent. 

These adsorbants are based on immobilised ligands, which have a high affinity for a particular analyte (Fig. 15.13). There are examples where antibodies have been raised to an analyte and then bound to the surface of a SPE matrix. Various types of chemistry permit this type of immobilisation and affinity chromatography is well established in biochemistry. With the proliferation with biotechnological products such as therapeutic peptides, the use of these types of columns for extraction may increase since they can be designed to be highly selective for such compounds. 

Labrou NE. Design and selection of ligands for affinity chromatography. Journal of Chromatography B 2003 790 67-78. 

C. King The Use of Coenzymes in Biochemical Reactors. - C. Wandrey, E Flaschel Process Development and Economical Aspects in Enzyme Engineering Acylase-L-Methionine System. - D.J. Graves, Yun-Tai Wu The Rational Design of Affinity Chromatography Separation Processes. 

Successful application of affinity chromatography requires careful design of experimental conditions. The essential components, which are outlined below, are (1) creation and preparation of a stationary matrix with immobilized ligand and (2) design of column development and eluting conditions. 

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