Affinity chromatography anti-carbohydrate antibodies

With the advent of affinity chromatography to isolate enzymes [7] it was possible to adapt the method for the isolation of antibodies [8], The purification of many types of anti-carbohydrate antibodies with specificity for carbohydrate residues of macromolecules has been achieved. The antibodies were isolated by affinity chromatography on adsorbents with proper ligands. The immunodeterminants of the antigens have been found to be mono- or disaccharide residues of the antigen. The anti-carbohydrate antibodies with unique properties are useful in a variety of medicinal and technological applications. In this article the emphasis is on polyclonal antibodies specifically the preparation, properties and uses of anti-carbohydrate antibodies. 

Antibodies with specificity for specific carbohydrate residues of antigens have been isolated from serum of rabbits immunized with carbohydrate containing antigens. Eighteen such antibodies were purified by affinity chromatography on adsorbents with ligands of carbohydrate residues. Electrofocusing results showed that all the antibodies occurred in multiprotein forms. A number of anti-carbohydrate antibodies have had useful applications. In the future additional advances with anti-carbohydrate antibodies should be made and these can lead to developments of new and improved products and processes. Some of the applications are summarized ... 

All the anti-carbohydrate antibodies purified by affinity chromatography are isomers of a different number of isoforms. The forms can be separated by electrofocusing and all have been found to have combining activity for the immunodeterminant of the same antigen by the method of coupled analysis of electrofocusing and agar diffusion [88], The isoforms of antimonosaccharide antibodies are shown in Fig. (48). 

Antibodies.— Antibodies to the milk oligosaccharide (40), viz. anti-Oacto- -difucohexaose) antibody, have been complexed with succinylated haemocyanin as part of a study of the affinity chromatography of carbohydrate-specific immunoglobulins. ... 

Fig. 1.—Affinity chromatography elution pattern for anti-rhamnose antibodies in immune serum from Streptococcus mutans. The arrows indicate the point of application of the serum and of 0.5 M L-rhamnose solution. The inset is an agar-diffusion pattern of the antibodies against the antigen. (Reprinted from Carbohydrate Research, Volume 124, J. H. Pazur, M. S. Erikson, M. Tay, and P. Z. Allen, pp. 253-263, copyright 1983, with permission from Elsevier Science Ltd., The Boulevard, Langford Lane, Kidlington 0X5 1GB, UK.)...

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