Adriamycin electrodes

Fig. 3.10 Square-wave voltammetry of adriamycin adsorbed on mercury electrode. A net response and its forward and backward components. The concentration of adriamycin is 1.72 x 10 " M and the supporting electrolyte is 0.9 M KNO3, pH 4.65. Adriamycin is accumulated during 30 s from unstirred solution, at —0.1 V. sw = 50 mV, / = 10 Hz and AE = —2 mV (reprinted from [190] with permission)...

Sqnare-wave voltammogram of antibiotic adriamycin adsorbed to the snrface of static mercnry drop electrode is shown in Fig. 3.10 [190]. It originates from the redaction of qninone gronp in the molecule. Figure 3.11 shows the dependence of the... 

These results are in agreement with those obtained with the thick-layer dsDNA-electrochemical biosensor. The clear separation of the adriamycin and 8-oxoGua peaks can be explained by the non-uniform coverage of the electrode surface by DNA and the adsorption of adriamycin [37] on the uncovered glassy carbon. This peak separation is... 

The electrochemical study of the in situ interaction of quercetin, adriamycin, DETA/NO and their metabolites with double-stranded DNA (dsDNA) immobilized on a glassy carbon electrode (GCE) surface. 

To study the interaction between adriamycin and DNA, a GCE (d — 6 mm) working electrode, a Pt wire counter electrode and a saturated calomel electrode (SCE) as reference electrode are used in a 5 ml one-compartment electrochemical cell. 

Further research for useful mediators led to the anthracycline antibiotic adria-mycin which serves as a novel mediator for the FNR or diaphorase (DP) catalyzed electrochemical reduction of NAD(P)+ [107]. This regeneration system has been satisfactory combined with NAD(P)H-dependent enzymatic reactions. The NADP+-dependent FNR/adriamycin system was coupled with GluDH. GluDH was entrapped together with FNR on the electrode surface. NADPH was efficiently... 

Altered chemical, physicochemical, and structural properties of damaged DNA are reflected in its redox behavior, which is utilized in numerous techniques of DNA damage detection. Electrochemical DNA-based biosensors have been used not only to detect but also to induce and control DNA damage at the electrode surface via electrochemical generation of the damaging (usually radical) species [13]. This way, chemicals and drugs such as niclosamide, adriamycin. 

Also, intercalated adriamycin has been used as hybridization label in a genosensor built by modifying a glassy carbon electrode (GCE) with multiwalled carbon nanotubes with carboxyl groups and Au-NPs [23], Differential pulse voltammetry (DPV) was utilized to monitor the DNA hybridization event. Under the optimal conditions, the increase of reduction peak current of adriamycin was linear with the logarithm of the concentration of the complementary oligonucleotides from 1.0 x 10 to 5.0 x 10 M with a detection limit of 3.5 x 10 M. 

In previous papers we have shown that anthracycline adriamycin (ADM) and its derivatives are strongly adsorbed on electrode surfaces and that the two-electron redox reaction of the anthraquinone moiety occurs through its semiquinone radical intermediate even in aqueous media. In addition, we have found characteristic reduction waves of molecular oxygen catalyzed by ADM and 7-deoxyadriamycinone (7-DADMN) adsorbed on basal-plane pyrolytic graphite electrode (BPGE) surfaces this report, we... 

Akpofure et al. used HPLC and electrochemical detection at an oxidative potential of 0.65 V to quantify daunorubicin and its metabolites in serum and plasma. Using a different method, Chaney and Baldwin determined Adriamycin concentrations in urine. The determination was performed by adsorption of the compound at a carbon paste electrode and then applying pulse voltammetric analysis at the resulting surface. 

In other applications, Anne et al. used electrochemical reduction to synthesize new daunorubicin analogs. Kano et al. studied the electrochemical properties of Adriamycin adsorbed on a mercury electrode, while Konse et al. studied the electrochemical properties of Adriamycin adsorbed on pyrolyte graphite electrodes modified by phospholipid membranes. This latter work has relevance to the interaction of Adriamycin with cell membranes. 

---