Adipocere, formation

Lake samples are more similar to the glacier samples than to fresh samples obviously adipocere formation is similar in glaciers and a deep alpine lake. 

Advanced decay Liquefaction of soft tissues, saponification of lipids, adipocere formation... 

In an initial study at the pasture site, replicate samples of dyed polyester, undyed wool, undyed cotton, and synthetic indigo-dyed denim were placed above (30 cm depth) and below (60 cm depth) cadavers and at 30 cm and 60 cm depth in control graves. When exhumed at 24 months, cadaver B was still actively decomposing with extensive adipocere formation at the base of the grave. This was confirmed by determination of carbon dioxide respiration rates for soil samples taken at the time of recovery (Wilson 2002). It was clear that decomposition of the cadaver had influenced the decomposition rates of the textiles depending on location in the grave after 24 months of burial (Table 7.6). 

Adipocere formation has been observed in a variety of burial environments, including lead-lined coffins (Mant 1987), peat bogs (Evershed 1992), ice glaciers (Mayer, Reiter, and Bereuter 1997), and submerged locations (Kahana et al. 1999), and consequently numerous principles governing its... 

Plastic coverings are often used to wrap the body before burial, both within a coffin or when placed directly in the soil. The use of plastic to cover bodies also greatly retards decomposition however, its affect on adipocere formation is variable. Some cases report extensive adipocere formation in bodies wrapped only in plastic (Manhein 1997), whereas experimental studies show that tissue wrapped in plastic and buried in soil will result in a semifluid mass of putrefied tissue but no adipocere formation unless clothing is also present (Forbes et al. 2005b). 

The formation of adipocere can occur in a vast range of burial environments and will proceed by the extraction of moisture from the internal tissues until they become desiccated and mummified (Ubelaker 1995). The dehydration of the internal organs as a result of adipocere formation accounts for the cessation of putrefaction and subsequently decomposition (Fiedler, Sch-nekenberger, and Graw 2004). In this way, adipocere formation can preserve human remains to an extent that can permit postmortem identification of the body (Poison et al. 1985). Frequently, adipocere formation and mummification will occur simultaneously, making the corpse extremely resistant to decomposition (Bereuter et al. 1996 Makristathis et al. 2002) (Figure 8.2). 

However, adipocere formation is not an end product, and eventually adipocere will also decompose. The factors necessary for decomposition of adipocere are not well documented. Similar to its formation, the decomposition of adipocere appears to be dependent on the surrounding burial environment (Fiedler et al. 2004). Early studies reported that an aerobic environment was necessary to decay adipocere, suggesting that adipocere would only decompose when a grave site was exposed to air or when an adipocerous body was exhumed to the soil surface (Evans 1963 Mant 1957, 1987). More recent studies suggest that the persistence of adipocere in a burial environment is related to the exclusion of gram positive bacteria, including species of Bacillus, Cellulomonas, and Nocardia, which are able to decompose adipocere (Pfeiffer et al. 1998). 

Bereuter, T. L., Lorbeer, E., Reiter, C., Seidler, H., and Unterdorfer, H. (1996). Postmortem alterations of human lipids—Part I Evaluation of adipocere formation and mummification by desiccation, in Human Mummies A Global Survey of Their Status and the Techniques of Conservation (K. Spindler, Ed.). New York Wien, 265-273. 

Kahana, T., Almog, J., Levy, J., Shmeltzer, E., Spier, Y., and Hiss, J. (1999). Marine taphonomy adipocere formation in a series of bodies recovered from a single shipwreck. /. Forensic Sci. 44, 897-901. 

Takatori, T. (1996). Investigations on the mechanism of adipocere formation and its relation to other biochemical reactions. Forensic Sci. Int. 80,49-61. 

Takatori, T. and Yamaoka, A. (1977a). The mechanism of adipocere formation I Identification and chemical properties of hydroxy fatty acids in adipocere. J. Forensic Sci. 9, 63-73. 

Takatori, T., Gotouda, H., Terazawa, K., Mizukami, K., and Nagao, M. (1987). The mechanism of experimental adipocere formation substrate specificity of microbial production of hydroxy and oxo fatty acids. Forensic Sci. Int. 35, 277-281. 

Figure 8.2 Decomposed porcine remains with partial mummification and adipocere formation.

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