Activity, definition rational

Rational design, 148-149, 152 Real-time assays, 83, 88 Receptor(s). See also Drug receptors affinity for, 6, 63 allosteric model of, 143 Clark s work, 3 classical model of, 44-45 concept of, 2-4 conformations, 13-14, 13 Of conservation equation for, 76 constitutive activity of, 49-51 coupling of, 27 definition of, 2 desensitization of, 34 efficacy for, 6... 

While the results of this work are encouraging, it is clear that the structural definition of mutant proteins of this type is critical to development of rational interpretation of the results if for no other reason than that the structural perturbation introduced is presumably greater than for simple point mutations. Moreover, it would be particularly interesting to compare the functional properties of mutants compared in this manner in assays involving protein-protein reactions relevant to the species of cytochrome c on which the mutagenesis is based. For example, comparison of the activities of wild-type yeast cytochrome c with that of a loop-insertion mutant modelled on a photosynthetic cytochrome c in the reaction with the photosynthetic reaction center could help define the structural elements involved in the cytochrome c binding domain for the reaction center. 

See, for example, Chap. 9 in K. Denbigh, The Principles of Chemical Equilibrium, Cambridge University Press, Cambridge, 1981. ThelUPAC recommendation for the symbol to represent rational activity coefficients is yx, which is not used in this book in order to make the distinction between solid solutions and aqueous solutions more evident. In strict chemical thermodynamics, however, all activity coefficients are based on the mole fraction scale, with the definition for aqueous species (Eq. 1.12) actually being a variant that reflects better the ionic nature of electrolyte solutions and the dominant contribution of liquid water to these mixtures. (See, for example, Chap. 2 inR. A. RobinsonandR. H. Stokes,Electrolyte Solutions, Butterworths, London, 1970.)... 

By current definition, proteome means simply the internal protein-world of the organism while genome represents its internal DNA world. This definition invokes the all-important issue of three-dimensional structures of proteins, which are in turn the basis of the rational design of drugs. It also invokes many other aspects of protein function, such as protein-protein and protein-drug interactions. Within such broad definition, many companies are now active in areas that can reasonably be described as proteomics. 

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