Acorn-NMR

NUTS, Acorn NMR, demo version from ftp.netcom. com/pub/wp/woodyc e-mail support acomnmr.com. 

Further confirmation of the structure of each extractable compound was performed on a JOEL ECX-400 NMR spectrometer operating at 400 MHz at Acorn NMR Incorporated (Livermore, CA, USA) after isolation using solid-phase extraction (SPE) followed by analytical HPLC purification. For SPE, a BondElut Cl 8 cartridge was washed with 0.1% TFA in methanol and preconditioned with 0.1% TFA in water before loading about 50 mL of the foil extract at approximately 3 mL/min. The retained compounds were eluted with 10 mL of 0.1% TFA in methanol, and the solvent was evaporated to dryness using the Biichi Rotavapor R-124 rotary evaporator. The residue was then redissolved in 0.2 mL methanol and rinsed with 0.8 mL of 0.1% TFA in purified water prior to HPLC... 

Perkin-Elmer Ltd Post Olfice Lane Beaconsfield Bucks HP91QA UK Acorn NMR Inc 46560 Fremont Blvd 418 Fremont CA 94538 USA NMR data processing... 

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