AAV, serotypes

The development of AAV-based gene-therapy vectors is presently focused on the analysis of the properties of the different AAV serotypes, especially the host range of the virion shells of the different serotypes. By packaging... 

Different AAV serotypes have shown remarkably different expression patterns because of differences in cell entry and intracellular activities (66, 67). Application of the dimerizer-inducible transcriptional regulatory system for AAV has allowed pharmacological regulation of heterologous gene expression in vivo (68). 

Rabinowitz, J. E. et al. (2002). Cross-packaging of a single adeno-associated virus (AAV) type 2 vector genome into multiple AAV serotypes enables transduction with broad specificity. J. Virol. 76, 791-801. 

Rutledge, E. A., Halbert, C. L. and Russell, D. W. (1998). Infectious clones and vectors derived from adeno-associated virus (AAV) serotypes other... 

It is important to note that these AAV serotype-dependent transduction efficiencies have been determined in vitro and in murine and canine tissues in vivo whether the same specificity occurs... 

Recombinant AAV serotype 2 vectors were developed for CFTR gene transfer. These vectors were designed to overcome the intrinsic packaging limitation of rAAV by fitting the relatively large coding sequence of CFTR (approximately 4.5 kb), with minimal promoter... 

Wang, C. et al. (2003). Recombinant AAV serotype 1 transduction efficiency and tropism in the murine brain. Gene Ther. 10(17), 1528-1534. 

Studies have demonstrated that AAV serotypes 1 and 2 are able to efficiently transduce the murine heart for up to 10 months following GFP transgene delivery (Du et al., 2004). Recently, we have found that intravenous delivery to the neonatal mouse of the newly characterized AAV serotype 9 results in /i-galactosidase expression levels that are up to 50-fold higher in cardiac tissue than those obtained using AAV1 (Fig. 9.1) (Pacak, submitted 2004). The... 

Table 2.1 Transduction efficiencies of AAV serotypes in different tissues... 

Thorsen F, Afione S, Huszthy PC, Tysnes BB, Svendsen A, Bjerkvig R, Kotin RM, Lonning PE, Hoover F. Adeno-associated virus (AAV) serotypes 2, 4 and 5 display similar transduction profiles and penetrate solid tumor tissue in models of human glioma. J Gene Med... 

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